Strain Data Sheet

RBRC00455

Strain Information

Image
BRC No.RBRC00455
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Skp2<tm1Kin>
Former Common nameSkp2 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development＞10世代　C57BL/6に戻し交配
Strain descriptionB6.129P2-Skp2<tm1Kin>. Skp2 gene knock-out mice. Homozygous mutant mice are viable, but show reduced growth rate and increased apoptosis of cells.
Colony maintenance繁殖成績が低い形態の異常・奇形がある
References
Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication.
K Nakayama, H Nagahama, Y A Minamishima, M Matsumoto, I Nakamichi, K Kitagawa, M Shirane, R Tsunematsu, T Tsukiyama, N Ishida, M Kitagawa, K Nakayama, S Hatakeyama
EMBO J., 19, 2069-2081 (2000). 10790373

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Skp2
MGI:1351663		S-phase kinase-associated protein 215Skp2<tm1Kin>
MGI:2179051		targeted mutation 1, Kei-ichi Nakayama
neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal apoptosis (MP:0001648)

  • abnormal cell proliferation (MP:0000350)

  • abnormal hepatocyte morphology (MP:0000607)

  • abnormal immunoglobulin V(D)J recombination (MP:0008755)

  • abnormal kidney morphology (MP:0002135)
    • more 7 phenotypes
  • abnormal testis morphology (MP:0001146)

  • decreased body weight (MP:0001262)

  • delayed wound healing (MP:0002908)

  • increased apoptosis (MP:0006042)

  • increased fibroblast apoptosis (MP:0013501)

  • polyploidy (MP:0004025)

  • postnatal growth retardation (MP:0001732)
    • Detailed phenotype data

    Ordering Information

    供与核酸PGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Skp2 genomic DNA
    Research application
    提供条件利用者は事前に寄託者の提供承諾書を得る。共同研究
    Depositor中山　敬一（国立大学法人九州大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data