Strain Data Sheet

RBRC00445

Strain Information

Image
BRC No.RBRC00445
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameC57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain development開発者:岡部勝先生機関名:大阪大学遺伝子情報実験センター
Strain description480 nmで励起すれば、体および精子の先体が光る。
Colony maintenance特になし。+N10?+F3。 トランスジーンはホモ型に固定されている。兄妹交配による維持。
References
Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitation.
Nakanishi T, Ikawa M, Yamada S, Toshimori K, Okabe M
Dev. Biol., 237, 222-231 (2001). 11518518

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal
    Research applicationFluorescent Proteins/lacZ System
    Reproductive Biology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 237, 222-231 (2001).非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
    Depositor岡部 勝(国立大学法人大阪大学)
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
    A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
    Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697
    Abe T, Nishimura H, Sato T, Suzuki H, Ogawa T, Suzuki T.
    Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes.
    Data Brief 33 106482(2020) 33241095
    Sanjo H, Yao T, Katagiri K, Sato T, Matsumura T, Komeya M, Yamanaka H, Yao M, Matsuhisa A, Asayama Y, Ikeda K, Kano K, Aoki J, Arita M, Ogawa T.
    Antioxidant vitamins and lysophospholipids are critical for inducing mouse spermatogenesis under organ culture conditions.
    FASEB J 34(7) 9480-9497(2020) 32474967
    Lin YN, Roy A, Yan W, Burns KH, Matzuk MM.
    Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis.
    Mol Cell Biol 27(19) 6794-805(2007) 17664285
    Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T.
    Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
    Proc Natl Acad Sci U S A 109(42) 16934-8(2012) 22984182
    Komeya M, Yamanaka H, Sanjo H, Yao M, Nakamura H, Kimura H, Fujii T, Sato T, Ogawa T.
    In vitro spermatogenesis in two-dimensionally spread mouse testis tissues.
    Reprod Med Biol 18(4) 362-369(2019) 31607796
    Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM.
    High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation.
    Sci Rep 9(1) 12618(2019) 31575926