Strain Information | |
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Image | |
BRC No. | RBRC00445 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb |
Former Common name | C57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | black [a/a B/B C/C] |
Strain development | 開発者:岡部勝先生機関名:大阪大学遺伝子情報実験センター |
Strain description | 480 nmで励起すれば、体および精子の先体が光る。 |
Colony maintenance | 特になし。+N10?+F3。 トランスジーンはホモ型に固定されている。兄妹交配による維持。 |
References | Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitation. Nakanishi T, Ikawa M, Yamada S, Toshimori K, Okabe M Dev. Biol., 237, 222-231 (2001). 11518518 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GFP | Green Fluorescent Protein (Aequorea victoria) | UN | mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide | ||||
GFP | Green Fluorescent Protein (Aequorea victoria) | UN | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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供与核酸 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal |
Research application | Fluorescent Proteins/lacZ System Reproductive Biology Research |
提供条件 | 利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 237, 222-231 (2001).非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 |
Depositor | 岡部 勝(国立大学法人大阪大学) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697 |
Abe T, Nishimura H, Sato T, Suzuki H, Ogawa T, Suzuki T. Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes. Data Brief 33 106482(2020) 33241095 |
Sanjo H, Yao T, Katagiri K, Sato T, Matsumura T, Komeya M, Yamanaka H, Yao M, Matsuhisa A, Asayama Y, Ikeda K, Kano K, Aoki J, Arita M, Ogawa T. Antioxidant vitamins and lysophospholipids are critical for inducing mouse spermatogenesis under organ culture conditions. FASEB J 34(7) 9480-9497(2020) 32474967 |
Lin YN, Roy A, Yan W, Burns KH, Matzuk MM. Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Mol Cell Biol 27(19) 6794-805(2007) 17664285 |
Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T. Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proc Natl Acad Sci U S A 109(42) 16934-8(2012) 22984182 |
Komeya M, Yamanaka H, Sanjo H, Yao M, Nakamura H, Kimura H, Fujii T, Sato T, Ogawa T. In vitro spermatogenesis in two-dimensionally spread mouse testis tissues. Reprod Med Biol 18(4) 362-369(2019) 31607796 |
Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM. High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation. Sci Rep 9(1) 12618(2019) 31575926 |