Strain Data Sheet

RBRC00445

Strain Information

Image
BRC No.RBRC00445
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameC57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The CAG and acr-EGFP plasmids were co-injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6NCr background.
Strain descriptionTransgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc].
References
Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitation.
Nakanishi T, Ikawa M, Yamada S, Toshimori K, Okabe M
Dev. Biol., 237, 222-231 (2001). 11518518

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal
    Research applicationFluorescent Proteins/lacZ System
    Reproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 237, 222-231 (2001).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
    A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
    Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697
    Abe T, Nishimura H, Sato T, Suzuki H, Ogawa T, Suzuki T.
    Time-course microarray transcriptome data of in vitro cultured testes and age-matched in vivo testes.
    Data Brief 33 106482(2020) 33241095
    Sanjo H, Yao T, Katagiri K, Sato T, Matsumura T, Komeya M, Yamanaka H, Yao M, Matsuhisa A, Asayama Y, Ikeda K, Kano K, Aoki J, Arita M, Ogawa T.
    Antioxidant vitamins and lysophospholipids are critical for inducing mouse spermatogenesis under organ culture conditions.
    FASEB J 34(7) 9480-9497(2020) 32474967
    Lin YN, Roy A, Yan W, Burns KH, Matzuk MM.
    Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis.
    Mol Cell Biol 27(19) 6794-805(2007) 17664285
    Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T.
    Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
    Proc Natl Acad Sci U S A 109(42) 16934-8(2012) 22984182
    Komeya M, Yamanaka H, Sanjo H, Yao M, Nakamura H, Kimura H, Fujii T, Sato T, Ogawa T.
    In vitro spermatogenesis in two-dimensionally spread mouse testis tissues.
    Reprod Med Biol 18(4) 362-369(2019) 31607796
    Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM.
    High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation.
    Sci Rep 9(1) 12618(2019) 31575926