Strain Data Sheet

RBRC00440

Strain Information

Image
BRC No.RBRC00440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gab1<tm1Thir>/ThirRbrc
Former Common nameGab1 Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearancegray
yellow
Strain development開発年：2000年　開発者：平野　俊夫先生機関名：大阪大学
Strain description
Colony maintenancehomozygous (Gab1-/-) は胎生致死の為、出生せず。
References
Role of Gab1 in heart, placenta, and skin development and growth factor- and cytokine-induced extracellular signal-regulated kinase mitogen-activated protein kinase activation.
M Itoh, Y Yoshida, K Nishida, M Narimatsu, M Hibi, T Hirano
Mol. Cell. Biol., 20, 3695-3704 (2000). 10779359

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gab1
MGI:108088		growth factor receptor bound protein 2-associated protein 18Gab1<tm1Thir>
MGI:1926435		targeted mutation 1, Toshio Hirano
  • autosomal recessive nonsyndromic hearing loss 26(MedGEN)
    • IRES internal ribosomal entry site (EMCV)8
    SA Splice acceptor8SA
    lacZ beta-galactosidase (E. coli)8
    neo neomycin resistance gene (E. coli)8

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal epidermis stratum granulosum morphology (MP:0001239)

  • abnormal hair follicle development (MP:0003704)

  • absent eyelids (MP:0001341)

  • decreased embryo size (MP:0001698)

  • decreased trophoblast giant cell number (MP:0001713)
    • more 12 phenotypes
  • dilated heart left ventricle (MP:0002753)

  • dilated heart right ventricle (MP:0002754)

  • early eyelid opening (MP:0003153)

  • hemopericardium (MP:0005244)

  • hemorrhage (MP:0001914)

  • lethality throughout fetal growth and development, complete penetrance (MP:0011099)

  • petechiae (MP:0008816)

  • placental labyrinth hypoplasia (MP:0001715)

  • small placenta (MP:0004259)

  • thin epidermis (MP:0001218)

  • thin ventricular wall (MP:0000280)

  • ventricular hypoplasia (MP:0000279)
    • Detailed phenotype data

    Ordering Information

    供与核酸SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Bgeo(E. coli lacZ, E. coli neo), mouse Gab1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。研究成果の公表にあたって謝辞の表明を必要とする。公表を前提とした学術研究に限る。
    共著を必要とする共同研究は寄託後1年間に限定する。
    利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場合は、寄託者と別途協議を行う。
    Depositor平野　俊夫（大阪大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Tokita S, Watanabe N, Hasegawa A, Funaya S, Miura K, Matoba S, Ogura A, Inoue K.
    Overexpression of Placenta-Specific Noncanonical Imprinted Genes Causes Placental Enlargement in Intersubspecific Hybrid Mice.
    Biol Reprod (2025) 41283858