Strain Data Sheet

RBRC00438

Strain Information

Image
BRC No.RBRC00438
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Map2k4<tm1Pngr>/Rbrc
Former Common nameSEK1 Knockout mice
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development1997年、仁科博史先生、トロント大学・オンタリオ癌研究所
Strain descriptionsek1(-/-)は胎生10〜12.5日で致死となる。継代はsek1(+/-)のへテロで行う。へテロマウスに異常は観察されていない。
Colony maintenance交配は、sek1(+/-)♂、C57BL/6♀で行って下さい。タイピングの必要有り。
References
Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3.
Nishina H, Fischer K D, Radvanyi L, Shahinian A, Hakem R, Rubie E A, Bernstein A, Mak T W, Woodgett J R, Penninger J M
Nature, 385, 350-353 (1997). 9002521

Defective liver formation and liver cell apoptosis in mice lacking the stress signaling kinase SEK1/MKK4.
Nishina H, Vaz C, Billia P, Nghiem M, Sasaki T, De la Pompa J L, Furlonger K, Paige C, Hui C, Fischer K D, Kishimoto H, Iwatsubo T, Katada T, Woodgett J R, Penninger J M
Development, 126, 505-516 (1999). 9876179

SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis.
Watanabe T, Nakagawa K, Ohata S, Kitagawa D, Nishitai G, Seo J, Tanemura S, Shimizu N, Kishimoto H, Wada T, Aoki J, Arai H, Iwatsubo T, Mochita M, Watanabe T, Satake M, Ito Y, Matsuyama T, Mak T W, Penninger J M, Nishina H, Katada T
Dev. Biol., 250, 332-347 (2002). 12376107

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Map2k4
MGI:1346869		mitogen-activated protein kinase kinase 411Map2k4<tm1Pngr>
MGI:1857536		targeted mutation 1, Josef M Penninger
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal liver development (MP:0000596)

  • anemia (MP:0001577)

  • embryonic lethality during organogenesis, complete penetrance (MP:0011098)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse mitogen activated protein kinase kinase 4 genomic DNA
    Research applicationCell Biology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。研究成果の公表にあたって謝辞の表明を必要とする。公表を前提とした学術研究に限る。
    分譲にあたっては寄託後、寄託者との共同研究とする。
    利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場合は、寄託者と別途協議を行う。
    Depositor仁科　博史（国立大学法人東京大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Sugiyama D, Kulkeaw K, Mizuochi C.
    TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts.
    Mech Dev 130(2-3) 195-206(2013) 23041440
    Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S.
    Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production.
    Biochem Biophys Res Commun 410(2) 301-6(2011) 21664343