Strain Data Sheet

RBRC00437

Strain Information

Image
BRC No.RBRC00437
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prnp<tm1Ito>/Rbrc
Former Common namePrnp-/- mouse, Prion protein (PrP) Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Takashi Yokoyama, National Institute of Animal Health and Shigeyoshi Itohara, RIKEN BSI in 2001.C57BL/6J background.
Strain description加齢に伴う後肢麻痺.
Colony maintenance特になし
ReferencesJ. Biol. Chem., 276, 11265-11271 (2001). 11152682
Nature, 400, 225-226 (1999). 10421360

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PrnPprion protein2PrnP<tm1Ito>targeted mutation 1, Shigeyoshi ItoharaPrP, PrPC, Sinc, +Prnp, CD230, PrPSc, Prn-i, Prn-p, PrP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse prion genomic DNA
Research applicationMouse Models for Human Disease
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nature. 1999 Jul 15;400(6741):225-6.
Depositor小野寺　節（国立大学法人東京大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H.
Cutting Edge: Brucella abortus exploits a cellular prion protein on intestinal M cells as an invasive receptor.
J Immunol 189(4) 1540-4(2012) 22772447