Strain Data Sheet

RBRC00437

Strain Information

Image
BRC No.RBRC00437
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prnp<tm1Ito>/Rbrc
Former Common namePrnp-/- mouse, Prion protein (PrP) Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Takashi Yokoyama, National Institute of Animal Health and Shigeyoshi Itohara, RIKEN BSI in 2001.C57BL/6J background.
Strain description加齢に伴う後肢麻痺.
Colony maintenance特になし
References
Prions prevent neuronal cell-line death.
C Kuwahara, A M Takeuchi, T Nishimura, K Haraguchi, A Kubosaki, Y Matsumoto, K Saeki, Y Matsumoto, T Yokoyama, S Itohara, T Onodera
Nature, 400(6741):225-226 (1999). 10421360

In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies.
T Yokoyama, K M Kimura, Y Ushiki, S Yamada, A Morooka, T Nakashiba, T Sassa, S Itohara
J. Biol. Chem., 276, 11265-11271 (2001). 11152682

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
PrnP
MGI:97769		prion protein2PrnP<tm1Ito>
MGI:3054245		targeted mutation 1, Shigeyoshi Itohara
  • Creutzfeldt Jacob disease(DisGeNET)

  • fatal familial insomnia(DisGeNET, MedGEN)

  • Gerstmann-Straussler-Scheinker syndrome(DisGeNET, MedGEN)
    • more 4 Diseases
  • Huntington disease-like 1(MedGEN)

  • inherited Creutzfeldt-Jakob disease(MedGEN)

  • kuru, susceptibility to(MedGEN)

  • spongiform encephalopathy with neuropsychiatric features(MedGEN)
    • neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • ataxia (MP:0001393)

  • tremors (MP:0000745)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse prion genomic DNA
    Research applicationMouse Models for Human Disease
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 400(6741):225-226 (1999).
    Depositor小野寺　節（国立大学法人東京大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H.
    Cutting Edge: Brucella abortus exploits a cellular prion protein on intestinal M cells as an invasive receptor.
    J Immunol 189(4) 1540-4(2012) 22772447