Strain Data Sheet

RBRC00408

Strain Information

Image
BRC No.RBRC00408
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gfap<tm1Ito>/ItoRbrc
Former Common nameGfap KO, GFAP-KO-clone 27
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain development1995年京都大学ウイルス研究所にて五味浩司先生と糸原重美先生によって作製された。
Strain descriptionアストリサイト特異的グリア繊維性酸性蛋白質を失損する。
Colony maintenance繁殖効率：A特になし
References
Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions.
H Gomi, T Yokoyama, K Fujimoto, T Ikeda, A Katoh, T Itoh, S Itohara
Neuron, 14, 29-41 (1995). 7826639

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gfap
MGI:95697		glial fibrillary acidic protein11Gfap<tm1Ito>
MGI:2183216		targeted mutation 1, Shigeyoshi Itohara
  • Alexander disease(DisGeNET, MedGEN)
    • lacZ beta-galactosidase (E. coli)11 endogenous start codon
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal excitatory postsynaptic potential (MP:0002912)

  • abnormal eye blink conditioning behavior (MP:0002807)

  • reduced long-term depression (MP:0001475)
    • Detailed phenotype data

    Ordering Information

    供与核酸E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse glial fibrillary acidic protein genomic DNA
    Research applicationNeurobiology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Neuron, 14, 29-41 (1995).
    Depositor糸原　重美（理化学研究所脳科学総合研究センター）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.
    Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP.
    Genes Dev 22(4) 489-98(2008) 18281462