Strain Data Sheet

RBRC00408

Strain Information

Image
BRC No.RBRC00408
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gfap<tm1Ito>/ItoRbrc
Former Common nameGfap KO, GFAP-KO-clone 27
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1995. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionGfap knockout mice. Immediately downstream of the endogenous start codon of Gfap was replaced with a cassette, containing a promoterless lacZ and pgk-neo. Gfap, glial fibrillary acidic protein is an intermediate filament protein specifically expressed in astrocytes. Homozygous mutant mice develop normally and not show any detectable abnormalities in the anatomy of the brain. But, exhibit a impairment of long-term depression (LTD) and PF-Purkinje cell synapses and eyeblink, suggested that the Gfap is required for communications between Bergmann glia and Purkinje cells.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
References
Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions.
Gomi H, Yokoyama T, Fujimoto K, Ikeda T, Katoh A, Itoh T, Itohara S
Neuron, 14, 29-41 (1995). 7826639

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gfapglial fibrillary acidic protein11Gfaptargeted mutation 1, Shigeyoshi Itohara
  • Alexander disease(DisGeNET, MedGEN)
  • lacZbeta-galactosidase (E. coli)11endogenous start codon
  • Alexander disease(DisGeNET, MedGEN)
  • neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)
  • Alexander disease(DisGeNET, MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal excitatory postsynaptic potential(MP:0002912)

  • abnormal eye blink conditioning behavior(MP:0002807)

  • reduced long-term depression(MP:0001475)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse glial fibrillary acidic protein genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 14, 29-41 (1995).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.
    Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP.
    Genes Dev 22(4) 489-98(2008) 18281462