Strain Data Sheet

RBRC00398

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC00398
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129P2-Mmp2<tm1Ito>/ItoRbrc
Former Common name	GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype
ES Cell line	E14 [129P2/OlaHsd]
Background strain	C57BL/6JJcl
Appearance	black
Strain development	1997年発表、糸原重美、伊藤　剛、京都大学ウイルス研究所
Strain description	ゲラチネースA（メタロプロテイネース2型）を完全に欠失する。軽度の発育不全を示す。
Colony maintenance	Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
References	J. Biol. Chem., 272, 22389-22392 (1997). 9278386

Health Report
Examination Date / Room / Rack	2024/01/09Room:3-ARack:B 2023/10/10Room:3-ARack:B 2023/07/10Room:3-ARack:B 2023/04/10Room:3-ARack:B 2023/01/10Room:3-ARack:B 2022/10/11Room:3-ARack:B 2022/09/05Room:3-ARack:B 2022/07/11Room:3-ARack:B

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Mmp2Matrix metalloproteinase 28Mmp2<tm1Ito>targeted mutation 1, Shigeyoshi Itohara72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)8neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information
供与核酸	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA
Research application	Cardiovascular Research Developmental Biology Research
提供条件	条件を付加する。研究成果の公表にあたって寄託者の指定する文献を引用する。J. Biol. Chem., 272, 22389-22392 (1997).
Depositor	糸原　重美（理化学研究所脳科学総合研究センター）
Strain Status	生体凍結胚凍結精子
Strain Availability	凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能生体マウスを1~3ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR- Genetic Background

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S. Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts. Am J Pathol 173(3) 781-91(2008) 18669618 Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H. Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy. Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097 Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A. Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction. Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562 Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T. Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging. Contrast Media Mol Imaging 2019 8908943(2019) 30723388 Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE. Matrix metalloproteinase 14 is required for fibrous tissue expansion. Elife 4 e09345(2015) 26390284 Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM. Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis. J Exp Med 203(4) 1007-19(2006) 16585265 Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H. Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2. Kidney Int 78(1) 69-78(2010) 20375993 Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S. Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice. Nephrol Dial Transplant 28(1) 55-62(2013) 23028104 Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N. Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway. Redox Biol 4 169-79(2015) 25574848 Bormann T, Maus R, Stolper J, Tort Tarrés M, Brandenberger C, Wedekind D, Jonigk D, Welte T, Gauldie J, Kolb M, Maus UA. Role of matrix metalloprotease-2 and MMP-9 in experimental lung fibrosis in mice. Respir Res 23(1) 180(2022) 35804363

BRC mice in Publications

Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts.
Am J Pathol 173(3) 781-91(2008) 18669618

Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H.
Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy.
Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097

Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A.
Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction.
Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562

Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T.
Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging.
Contrast Media Mol Imaging 2019 8908943(2019) 30723388

Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.
Matrix metalloproteinase 14 is required for fibrous tissue expansion.
Elife 4 e09345(2015) 26390284

Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.
J Exp Med 203(4) 1007-19(2006) 16585265

Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2.
Kidney Int 78(1) 69-78(2010) 20375993

Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice.
Nephrol Dial Transplant 28(1) 55-62(2013) 23028104

Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N.
Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.
Redox Biol 4 169-79(2015) 25574848

Bormann T, Maus R, Stolper J, Tort Tarrés M, Brandenberger C, Wedekind D, Jonigk D, Welte T, Gauldie J, Kolb M, Maus UA.
Role of matrix metalloprotease-2 and MMP-9 in experimental lung fibrosis in mice.
Respir Res 23(1) 180(2022) 35804363

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications