Strain Information | |
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Image | |
BRC No. | RBRC00361 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129-Mapk14<tm1Mka>(118)/Rbrc |
Former Common name | C57BL/6-TgH(p38)/118 |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6J |
Appearance | black [a/a] |
Strain development | This B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute. |
Strain description | Activity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression. |
Colony maintenance | The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14). |
References | Cell, 102, 221-231(2000). 10943842 J. Biol. Chem., 277, 37896-37903 (2002). 12138127 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mapk14mitogen activated protein kinase 1417Mapk14<tm1Mka>targeted mutation 1, Michael KarinCrk1, Csbp1, CSBP2, Mxi2, p38, p38 MAP Kinase, p38-alpha, PRKM14, PRKM15; p38 alpha MAP kinase Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17neo; neomycin; |
Ordering Information | |
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供与核酸 | E. coli neo, mouse p38 genomic DNA |
Research application | |
提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。 研究成果の公表にあたって謝辞の表明を必要とする。 公表を前提とした学術研究に限る。共著を必要とする共同研究は寄託後2年間に限定する。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。 |
Depositor | 須藤 龍彦(独立行政法人理化学研究所) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ohta M, Tateishi K, Kanai F, Ueha S, Guleng B, Washida M, Tanaka Y, Ijichi H, Ikenoue T, Sata M, Sudo T, Shiina S, Kawabe T, Matsushima K, Omata M. Reduced p38 mitogen-activated protein kinase in donor grafts accelerates acute intestinal graft-versus-host disease in mice. Eur J Immunol 35(7) 2210-21(2005) 15971269 |