Strain Data Sheet

RBRC00361

Strain Information

Image
BRC No.RBRC00361
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129-Mapk14<tm1Mka>(118)/Rbrc
Former Common nameC57BL/6-TgH(p38)/118
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6J
Appearanceblack [a/a]
Strain developmentThis B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.
Strain descriptionActivity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression.
Colony maintenanceThe mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14).
References
Requirement for p38alpha in erythropoietin expression: a role for stress kinases in erythropoiesis.
Tamura K, Sudo T, Senftleben U, Dadak A M, Johnson R, Karin M
Cell, 102, 221-231(2000). 10943842

Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity.
Takanami-Ohnishi Y, Amano S, Kimura S, Asada S, Utani A, Maruyama M, Osada H, Tsunoda H, Irukayama-Tomobe Y, Goto K, Karin M, Sudo T, Kasuya Y
J. Biol. Chem., 277, 37896-37903 (2002). 12138127

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mapk14mitogen-activated protein kinase 1417Mapk14targeted mutation 1, Michael Karin
neoneomycin resistance gene (E. coli)17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal erythropoiesis(MP:0000245)

  • abnormal placental labyrinth vasculature morphology(MP:0008803)

  • abnormal response to cardiac infarction(MP:0000343)

  • anemia(MP:0001577)

  • decreased myocardial infarct size(MP:0003038)
  • more 7 phenotypes
  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, incomplete penetrance(MP:0011108)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • pale liver(MP:0000603)

  • pale yolk sac(MP:0001722)

  • pallor(MP:0003717)

  • small liver(MP:0000601)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse p38 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
    The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTatsuhiko Sudo (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ohta M, Tateishi K, Kanai F, Ueha S, Guleng B, Washida M, Tanaka Y, Ijichi H, Ikenoue T, Sata M, Sudo T, Shiina S, Kawabe T, Matsushima K, Omata M.
    Reduced p38 mitogen-activated protein kinase in donor grafts accelerates acute intestinal graft-versus-host disease in mice.
    Eur J Immunol 35(7) 2210-21(2005) 15971269