Strain Data Sheet



BRC No.RBRC00306
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S1-Tnf<tm1Ljo>/Rbrc
Former Common nameTNF.K.O
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development1996年、Dr. Michael W.Marino、Ludwig Institute for Cancer Research
Strain description(1) TNFノックアウトマウスは健康に発育し、子孫を繁殖させる。(2) TNFの生体に及ぼす影響をin vivoにて検討できる。
Colony maintenanceホモ同士の交配にて継代が可能。
ReferencesProc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnftumor necrosis factor17Tnf<tm1Ljo>targeted mutation 1, Lioyd J Oldtumor necrosis factor alpha, DIF, Tnfa, TNFSF2, TNFalpha, TNF-alpha, TNF alpha, Tnfsf1a, tumor necrosis factor-alpha

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


供与核酸mouse tumor necrosis factor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationDevelopmental Biology Research
Immunology and Inflammation Research
Inquiries regarding the use of the BIOLOGICAL RESOURCE for commercial purposes including research use by for-profit companies should be directed to:
Dr. Par Olsson
Director, Intellectual Property
Ludwig Institute for Cancer Research
666 Third Avenue
New York, NY 10017
Tel. +1-212-450-1500
Fax. +1-212-450-1535

Ownership of modifications and derivatives including crossed progeny of the BIOLOGICAL RESOURCE will be determined in accordance with applicable law governing inventorship and the creative input of the parties and the Recipient agrees to report any such progeny to LICR. The RIKEN BRC, the LICR and Recipient agree that no party will license or otherwise commercialize any such invention in the absence of an agreement to be negotiated in good faith by the parties hereto, providing for, inter alia, the sharing of royalty income.
DepositorGerd Ritter(Ludwig Institute for Cancer Research)
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data