Strain Data Sheet

RBRC00306

Strain Information

Image
BRC No.RBRC00306
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S1-Tnf<tm1Ljo>/Rbrc
Former Common nameTNF.K.O
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development1996年、Dr. Michael W.Marino、Ludwig Institute for Cancer Research
Strain description(1)　TNFノックアウトマウスは健康に発育し、子孫を繁殖させる。(2)　TNFの生体に及ぼす影響をin vivoにて検討できる。
Colony maintenanceホモ同士の交配にて継代が可能。
References
Characterization of tumor necrosis factor-deficient mice.
Marino M W, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old L J
Proc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798		tumor necrosis factor17Tnf<tm1Ljo>
MGI:1860250		targeted mutation 1, Lioyd J Old
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Peyer's patch T cell area morphology (MP:0002392)

  • abnormal Peyer's patch follicle morphology (MP:0002389)

  • abnormal cerebral cortex morphology (MP:0000788)

  • abnormal chemokine secretion (MP:0008722)

  • abnormal cytokine secretion (MP:0003009)
    • more 33 phenotypes
  • abnormal follicular dendritic cell physiology (MP:0008197)

  • abnormal long-term spatial reference memory (MP:0008432)

  • abnormal lung morphology (MP:0001175)

  • abnormal lymph node cell ratio (MP:0008828)

  • abnormal motor coordination/balance (MP:0001516)

  • abnormal nervous system physiology (MP:0003633)

  • abnormal neutrophil physiology (MP:0002463)

  • abnormal spleen B cell follicle morphology (MP:0008470)

  • abnormal susceptibility to infection (MP:0001793)

  • absent follicular dendritic cells (MP:0008201)

  • absent spleen germinal center (MP:0008474)

  • decreased IgG level (MP:0001805)

  • decreased Peyer's patch number (MP:0008133)

  • decreased apoptosis (MP:0006043)

  • decreased circulating tumor necrosis factor level (MP:0008554)

  • decreased immunoglobulin level (MP:0002460)

  • decreased spleen white pulp amount (MP:0008479)

  • decreased susceptibility to autoimmune disorder (MP:0005351)

  • decreased susceptibility to bacterial infection (MP:0002411)

  • decreased susceptibility to endotoxin shock (MP:0008734)

  • impaired coordination (MP:0001405)

  • increased interferon-gamma secretion (MP:0008566)

  • increased leukocyte cell number (MP:0000218)

  • increased lymphocyte cell number (MP:0005013)

  • increased neutrophil cell number (MP:0000219)

  • increased susceptibility to bacterial infection (MP:0002412)

  • increased susceptibility to fungal infection (MP:0005399)

  • increased susceptibility to parasitic infection (MP:0005027)

  • liver/biliary system phenotype (MP:0005370)

  • lung inflammation (MP:0001861)

  • skin inflammation (MP:0004947)

  • skin lesions (MP:0001212)

  • small Peyer's patches (MP:0008135)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse tumor necrosis factor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationDevelopmental Biology Research
    Immunology and Inflammation Research
    covid19_onset
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。研究成果の公表にあたって謝辞の表明を必要とする。Inquiries regarding the use of the BIOLOGICAL RESOURCE for commercial purposes including research use by for-profit companies should be directed to:
    Dr. Par Olsson
    Director, Intellectual Property
    Ludwig Institute for Cancer Research
    666 Third Avenue
    New York, NY 10017
    Tel. +1-212-450-1500
    Fax. +1-212-450-1535

    Ownership of modifications and derivatives including crossed progeny of the BIOLOGICAL RESOURCE will be determined in accordance with applicable law governing inventorship and the creative input of the parties and the Recipient agrees to report any such progeny to LICR. The RIKEN BRC, the LICR and Recipient agree that no party will license or otherwise commercialize any such invention in the absence of an agreement to be negotiated in good faith by the parties hereto, providing for, inter alia, the sharing of royalty income.
    DepositorGerd Ritter（Ludwig Institute for Cancer Research）
    Strain Status凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data