Strain Data Sheet

RBRC00305

Strain Information

Image
BRC No.RBRC00305
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc
Former Common nameC57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B
H-2 Haplotype
ES Cell lineW9.5/D3 for TNF alpha, CCE [129/Sv] for relA
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain development愛知がんセンター・土井貴弘（1997）。RelAノックアウトマウスとTNFノックアウトマウスとの交配により作出。
Strain descriptionTnf遺伝子およびRelA遺伝子をノックアウトしたマウス。RelA遺伝子：リンパ球の発生に関わる因子として発見されたNF-kBファミリーの一つ。全身でくまなく発現している。RelAのみホモノックアウトマウスは胎生期13-14日頃に死亡するが、Tnf/RelAダブルミュータントマウスは生存可能。
Colony maintenanceTNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type
References
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Doi T S, Takahashi T, Taguchi O, Azuma T, Obata Y
J. Exp. Med., 185, 953-961 (1997). 9120401

Characterization of tumor necrosis factor-deficient mice.
Marino M W, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old L J
Proc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320

Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality.
Doi T S, Marino M W, Takahashi T, Yoshida T, Sakakura T, Old L J, Obata Y
Proc. Natl. Acad. Sci. USA, 96, 2994-2999 (1999). 10077625

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798		tumor necrosis factor17Tnf<tm1Ljo>
MGI:1860250		targeted mutation 1, Lioyd J Old
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)
    Rela
    MGI:103290    		v-rel reticuloendotheliosis viral oncogene homolog A (avian)19Rela<tm1Yuo>
    MGI:1860444    		targeted mutation 1, Yuichi Obata
  • mucocutaneous ulceration, chronic(MedGEN)
    • neo neomycin resistance gene (E. coli)19 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Peyer's patch T cell area morphology (MP:0002392)

  • abnormal Peyer's patch follicle morphology (MP:0002389)

  • abnormal Peyer's patch germinal center morphology (MP:0002391)

  • abnormal cerebral cortex morphology (MP:0000788)

  • abnormal chemokine secretion (MP:0008722)
    • more 47 phenotypes
  • abnormal cytokine secretion (MP:0003009)

  • abnormal follicular dendritic cell physiology (MP:0008197)

  • abnormal hepatocyte morphology (MP:0000607)

  • abnormal liver morphology (MP:0000598)

  • abnormal long-term spatial reference memory (MP:0008432)

  • abnormal lung morphology (MP:0001175)

  • abnormal lymph node cell ratio (MP:0008828)

  • abnormal lymphocyte physiology (MP:0003945)

  • abnormal motor coordination/balance (MP:0001516)

  • abnormal myogenesis (MP:0000729)

  • abnormal nervous system physiology (MP:0003633)

  • abnormal neutrophil physiology (MP:0002463)

  • abnormal spleen B cell follicle morphology (MP:0008470)

  • abnormal susceptibility to infection (MP:0001793)

  • absent follicular dendritic cells (MP:0008201)

  • absent spleen germinal center (MP:0008474)

  • decreased B cell proliferation (MP:0005093)

  • decreased IgA level (MP:0001807)

  • decreased IgG level (MP:0001805)

  • decreased IgG1 level (MP:0008495)

  • decreased Peyer's patch number (MP:0008133)

  • decreased T cell proliferation (MP:0005095)

  • decreased apoptosis (MP:0006043)

  • decreased circulating tumor necrosis factor level (MP:0008554)

  • decreased double-positive T cell number (MP:0005092)

  • decreased immunoglobulin level (MP:0002460)

  • decreased skeletal muscle fiber diameter (MP:0009402)

  • decreased spleen white pulp amount (MP:0008479)

  • decreased susceptibility to autoimmune disorder (MP:0005351)

  • decreased susceptibility to bacterial infection (MP:0002411)

  • decreased susceptibility to endotoxin shock (MP:0008734)

  • impaired coordination (MP:0001405)

  • increased interferon-gamma secretion (MP:0008566)

  • increased leukocyte cell number (MP:0000218)

  • increased lymphocyte cell number (MP:0005013)

  • increased neutrophil cell number (MP:0000219)

  • increased skeletal muscle fiber number (MP:0009405)

  • increased susceptibility to bacterial infection (MP:0002412)

  • increased susceptibility to fungal infection (MP:0005399)

  • increased susceptibility to parasitic infection (MP:0005027)

  • lethality throughout fetal growth and development, complete penetrance (MP:0011099)

  • liver/biliary system phenotype (MP:0005370)

  • lung inflammation (MP:0001861)

  • lymph node hypoplasia (MP:0008101)

  • skin inflammation (MP:0004947)

  • skin lesions (MP:0001212)

  • small Peyer's patches (MP:0008135)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tnf genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse Rela genomic DNA
    Research applicationApoptosis Research
    Cancer Research
    Cardiovascular Research
    Developmental Biology Research
    Diabetes and Obesity Research
    Endocrine Deficiency Research
    Hematological Research
    Immunology and Inflammation Research
    Internal/Organ Research
    Neurobiology Research
    covid19_onset
    covid19_progression
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Proc Natl Acad Sci USA., 96, 2994-2999 (1999). (1)　会社等が本件リソースの譲渡を希望する場合は、ラドウィックがん研究所にその旨問い合わせる。Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100
    (2)　本件リソースの特許権ならびに知的所有権は寄託者とラドウィックがん研究所が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合は、事前に寄託者とラドウィックがん研究所に連絡し、了解を得る。
    Depositor土井　貴裕（理化学研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month May 2009

    BRC mice in Publications

    No Data