RBRC00305

Information1

Image
BRC No.RBRC00305
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc
Former Common nameC57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B
H-2 Haplotype
ES Cell lineW9.5/D3 for TNF alpha, CCE [129/Sv] for relA
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentProduced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997.
Strain descriptionTNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo.
Colony maintenanceTNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type
ReferencesProc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320
Proc. Natl. Acad. Sci. USA, 96, 2994-2999 (1999). 10077625
J. Exp. Med., 185, 953-961 (1997). 9120401

Health Report

Examination Date / Room / Rack2012/05/07Room:2-検Rack:M
2012/03/05Room:2-検Rack:M
2012/01/10Room:2-検Rack:M
2011/11/07Room:2-検Rack:M
2011/09/05Room:2-検Rack:M
2011/07/04Room:2-検Rack:M
2011/04/25Room:2-検Rack:M
2011/03/07Room:2-検Rack:M

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tnftumor necrosis factor17Tnf<tm1Ljo>targeted mutation 1, Lioyd J Old

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Relav-rel reticuloendotheliosis viral oncogene homolog A19Rela<tm1Yuo>targeted mutation 1, Yuichi Obata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)19herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tnf genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse Rela genomic DNA
Research applicationApoptosis Research
Cancer Research
Cardiovascular Research
Developmental Biology Research
Diabetes and Obesity Research
Endocrine Deficiency Research
Hematological Research
Immunology and Inflammation Research
Internal/Organ Research
Neurobiology Research
covid19_onset
covid19_progression
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 96, 2994-2999 (1999). The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100
Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice.
DepositorTakahiro Doi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month May 2009

BRC mice in Publications

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