Strain Data Sheet

RBRC00305

Strain Information

Image
BRC No.RBRC00305
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc
Former Common nameC57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B
H-2 Haplotype
ES Cell lineW9.5/D3 for TNF alpha, CCE [129/Sv] for relA
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentProduced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997.
Strain descriptionTNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo.
Colony maintenanceTNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type
References
Characterization of tumor necrosis factor-deficient mice.
M W Marino, A Dunn, D Grail, M Inglese, Y Noguchi, E Richards, A Jungbluth, H Wada, M Moore, B Williamson, S Basu, L J Old
Proc. Natl. Acad. Sci. USA, 94, 8093-8098 (1997). 9223320

NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
T S Doi, T Takahashi, O Taguchi, T Azuma, Y Obata
J. Exp. Med., 185, 953-961 (1997). 9120401

Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality.
T S Doi, M W Marino, T Takahashi, T Yoshida, T Sakakura, L J Old, Y Obata
Proc. Natl. Acad. Sci. USA, 96, 2994-2999 (1999). 10077625

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798		tumor necrosis factor17Tnf<tm1Ljo>
MGI:1860250		targeted mutation 1, Lioyd J Old
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)
    Rela
    MGI:103290    		v-rel reticuloendotheliosis viral oncogene homolog A (avian)19Rela<tm1Yuo>
    MGI:1860444    		targeted mutation 1, Yuichi Obata
  • mucocutaneous ulceration, chronic(MedGEN)
    • neo neomycin resistance gene (E. coli)19 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal myogenesis (MP:0000729)

  • decreased IgG level (MP:0001805)

  • decreased circulating tumor necrosis factor level (MP:0008554)

  • decreased skeletal muscle fiber diameter (MP:0009402)

  • increased skeletal muscle fiber number (MP:0009405)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tnf genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse Rela genomic DNA
    Research applicationApoptosis Research
    Cancer Research
    Cardiovascular Research
    Developmental Biology Research
    Diabetes and Obesity Research
    Endocrine Deficiency Research
    Hematological Research
    Immunology and Inflammation Research
    Internal/Organ Research
    Neurobiology Research
    covid19_onset
    covid19_progression
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 96, 2994-2999 (1999). The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100
    Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice.
    DepositorTakahiro Doi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month May 2009

    BRC mice in Publications

    No Data