Strain Data Sheet

RBRC00301

Strain Information

Image
BRC No.RBRC00301
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Rela<tm1Yuo>/2Rbrc
Former Common nameRelA KO, RKO-2
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack
Strain development1996年、土井貴裕先生、愛知がんセンターホモが胎生致死の為、ヘテロ（RelA+/-)同士にて、交配し継代する。
Strain description胎生14.5日において致死となる。RKO-2および-3とは発現様式・組み換え様式も同一であるが、起源のES細胞が異なる.
Colony maintenance
References
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Doi T S, Takahashi T, Taguchi O, Azuma T, Obata Y
J. Exp. Med., 185, 953-961 (1997). 9120401

Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality.
Doi T S, Marino M W, Takahashi T, Yoshida T, Sakakura T, Old L J, Obata Y
Proc. Natl. Acad. Sci. USA, 96, 2994-2999 (1999). 10077625

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rela
MGI:103290		v-rel reticuloendotheliosis viral oncogene homolog A (avian)19Rela<tm1Yuo>
MGI:1860444		targeted mutation 1, Yuichi Obata
  • mucocutaneous ulceration, chronic(MedGEN)
    • neo neomycin resistance gene (E. coli)19 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal hepatocyte morphology (MP:0000607)

  • abnormal liver morphology (MP:0000598)

  • abnormal lymphocyte physiology (MP:0003945)

  • abnormal myogenesis (MP:0000729)

  • decreased B cell proliferation (MP:0005093)
    • more 5 phenotypes
  • decreased IgA level (MP:0001807)

  • decreased IgG1 level (MP:0008495)

  • decreased T cell proliferation (MP:0005095)

  • decreased double-positive T cell number (MP:0005092)

  • lethality throughout fetal growth and development, complete penetrance (MP:0011099)
    • Detailed phenotype data

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse RelA genomic DNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。J Exp Med., 185, 953-961 (1997). 本件リソースの特許権ならびに知的所有権は寄託者が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合は、事前に寄託者に連絡し、了解を得る。
    Depositor土井　貴裕（理化学研究所）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data