Strain Data Sheet

RBRC00267

Strain Information

Image
BRC No.RBRC00267
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb
Former Common nameC57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131
H-2 Haplotype
ES Cell line
Background strainInter- Substrain hybrid between C57BL/6Cr Slc and C57BL/6J Jcl
Appearanceblack [a/a]
Strain development大阪大学微生物病研究所・岡部 勝先生。C57BL/6受精卵へTgのインジェクションにより作出。C57BL/6背景(C57BL/6JJclとC57BL/6NCrSlcの混合背景)。
Strain descriptionオワンクラゲの蛍光蛋白を全身の細胞で発現する遺伝子導入マウス。480 nmの励起光のもとで全身が緑色の蛍光を発するので別名「グリーンマウス」とも呼ばれる。受精卵から細胞分裂により個体が形成される過程で細胞がどのように分化して運命が決定されていくのか、その細胞系譜を生体の中で追跡するのに有用な系統。発生研究、がん研究、再生医学研究の分野で利用される。トランスジーンの挿入位置は14D1。
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc]
References
Green fluorescent protein (GFP) as a vital marker in mammals.
Ikawa M, Yamada S, Nakanishi T, Okabe M
Curr. Top Dev. Biol., 44, 1-20 (1999). 9891875

'Green mice' as a source of ubiquitous green cells.
Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y
FEBS Lett., 407, 313-319 (1997). 9175875

Efficient selection of transgenic mouse embryos using EGFP as a marker gene.
Kato M, Yamanouchi K, Ikawa M, Okabe M, Naito K, Tojo H
Mol. Reprod. Dev., 54, 43-8 (1999). 10423296

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M
Genomics, 80, 564-574 (2002). 12504848

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:G
2024/05/27Room:4-BRack:G
2024/02/26Room:4-BRack:G
2023/11/27Room:4-BRack:G
2023/08/28Room:4-BRack:G
2023/05/29Room:4-BRack:G
2023/02/27Room:4-BRack:G

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype dataPhenopub Link

    Ordering Information

    供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
    Research applicationCell Biology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Reproductive Biology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。FEBS Lett., 407, 313-319 (1997). 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
    Depositor岡部 勝(国立大学法人大阪大学)
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを3~6ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2012

    BRC mice in Publications

    Tada M, Shinohara Y, Kato I, Hiraga K, Aizawa T, Demura M, Mori Y, Shinoda H, Mizuguchi M, Kawano K.
    Preparation and observation of fresh-frozen sections of the green fluorescent protein transgenic mouse head.
    Acta Histochem Cytochem 39(2) 31-4(2006) 17375207
    Ooka H, Kanda S, Okazaki H, Suzuki H, Mishima K, Saito I, Yagi M, Tomoda K, Nishiyama T.
    Characterization of side population (SP) cells in murine cochlear nucleus.
    Acta Otolaryngol 132(7) 693-701(2012) 22667338
    Kageyama S, Liu H, Nagata M, Aoki F.
    The role of ETS transcription factors in transcription and development of mouse preimplantation embryos.
    Biochem Biophys Res Commun 344(2) 675-9(2006) 16630543
    Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
    Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification of a Persistent Stem Cell Type.
    Biol Reprod 94(6) 122(2016) 27122635
    Pritchard S, Wick HC, Slonim DK, Johnson KL, Bianchi DW.
    Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung.
    Biol Reprod 87(2) 42(2012) 22674387
    Matoba S, Ogura A.
    Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice.
    Biol Reprod 84(4) 631-8(2011) 21076079
    Fujiki Y, Johnson KL, Peter I, Tighiouart H, Bianchi DW.
    Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers.
    Biol Reprod 81(1) 26-32(2009) 19279322
    Ohmura M, Ogawa T, Ono M, Dezawa M, Hosaka M, Kubota Y, Sawada H.
    Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal.
    Biol Reprod 68(6) 2304-13(2003) 12606404
    Ogawa T, Ohmura M, Yumura Y, Sawada H, Kubota Y.
    Expansion of murine spermatogonial stem cells through serial transplantation.
    Biol Reprod 68(1) 316-22(2003) 12493728
    Tsujigiwa H, Nishizaki K, Teshima T, Takeda Y, Yoshinobu J, Takeuchi A, Orita Y, Sugata Y, Nagatsuka H, Nagai N.
    The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium.
    Brain Res 1052(1) 10-5(2005) 15996641
    Muramatsu M, Yamamoto S, Osawa T, Shibuya M.
    Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth.
    Cancer Res 70(20) 8211-21(2010) 20924106
    Joo SY, Cho KA, Jung YJ, Kim HS, Park SY, Choi YB, Hong KM, Woo SY, Seoh JY, Ryu KH.
    Bioimaging for the monitoring of the in vivo distribution of infused mesenchymal stem cells in a mouse model of the graft-versus-host reaction.
    Cell Biol Int 35(4) 417-21(2011) 21062262
    Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
    Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
    Cell Biol Int 33(7) 772-7(2009) 19427913
    Ju SY, Cho KA, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
    Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
    Cell Biol Int 33(1) 31-5(2009) 18992833
    Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Mimura O, Taguchi A, Matsuyama T.
    Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke.
    Cell Death Differ 19(5) 756-67(2012) 22052192
    Takanori Shinotsuka, Tsuyoshi Miyazawa, Keiko Karasawa, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
    Stimulated Raman scattering microscopy reveals a unique and steady nature of brain water dynamics
    Cell Reports Methods 3 100519(2023)
    Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
    Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.
    Cell Stem Cell 14(2) 217-27(2014) 24506885
    Pritchard S, Peter I, Johnson KL, Bianchi DW.
    The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon.
    Chimerism 3(3) 59-64(2012) 23128065
    Jeon HJ, Choi JH, Jung IH, Park JG, Lee MR, Lee MN, Kim B, Yoo JY, Jeong SJ, Kim DY, Park JE, Park HY, Kwack K, Choi BK, Kwon BS, Oh GT.
    CD137 (4-1BB) deficiency reduces atherosclerosis in hyperlipidemic mice.
    Circulation 121(9) 1124-33(2010) 20176988
    Kiguchi N, Kobayashi Y, Saika F, Kishioka S.
    Epigenetic upregulation of CCL2 and CCL3 via histone modifications in infiltrating macrophages after peripheral nerve injury.
    Cytokine 64(3) 666-72(2013) 24135048
    Aoki H, Yamada Y, Hara A, Kunisada T.
    Two distinct types of mouse melanocyte: differential signaling requirement for the maintenance of non-cutaneous and dermal versus epidermal melanocytes.
    Development 136(15) 2511-21(2009) 19553284
    Choi JB, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H.
    Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta cells.
    Diabetologia 46(10) 1366-74(2003) 12898006
    Hiratsuka S, Tomita T, Mishima T, Matsunaga Y, Omori T, Ishibashi S, Yamaguchi S, Hosogane T, Watarai H, Omori-Miyake M, Yamamoto T, Shibata N, Watanabe A, Aburatani H, Tomura M, High KA, Maru Y.
    Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung.
    EMBO Mol Med 10(7) (2018) 29930175
    Kawase Y, Yanagi Y, Takato T, Fujimoto M, Okochi H.
    Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth.
    Exp Cell Res 295(1) 194-203(2004) 15051502
    Unno A, Kachi S, Batanova TA, Ohno T, Elhawary N, Kitoh K, Takashima Y.
    Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver.
    Exp Parasitol 134(2) 160-4(2013) 23538031
    Shimizukawa R, Sakata A, Hirose M, Takahashi A, Iseki H, Liu Y, Kunita S, Sugiyama F, Yagami K.
    Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.
    Genesis 42(1) 47-52(2005) 15830376
    Inoue S, Ishikawa K, Nakada K, Sato A, Miyoshi H, Hayashi J.
    Suppression of disease phenotypes of adult mito-mice carrying pathogenic mtDNA by bone marrow transplantation.
    Hum Mol Genet 15(11) 1801-7(2006) 16613898
    Hirata T, Osuga Y, Yoshino O, Hirota Y, Harada M, Takemura Y, Morimoto C, Koga K, Yano T, Tsutsumi O, Taketani Y.
    Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.
    Hum Reprod 20(8) 2092-6(2005) 15831509
    Nishio N, Ito S, Suzuki H, Isobe K.
    Antibodies to wounded tissue enhance cutaneous wound healing.
    Immunology 128(3) 369-80(2009) 20067537
    Tomida D, Nishiguchi KM, Kataoka K, Yasuma TR, Iwata E, Uetani R, Kachi S, Terasaki H.
    Suppression of choroidal neovascularization and quantitative and qualitative inhibition of VEGF and CCL2 by heparin.
    Invest Ophthalmol Vis Sci 52(6) 3193-9(2011) 21296829
    Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H.
    The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization.
    Invest Ophthalmol Vis Sci 52(3) 1431-8(2011) 21051720
    Kobayashi Y, Kiguchi N, Fukazawa Y, Saika F, Maeda T, Kishioka S.
    Macrophage-T cell interactions mediate neuropathic pain through the glucocorticoid-induced tumor necrosis factor ligand system.
    J Biol Chem 290(20) 12603-13(2015) 25787078
    Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A.
    Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer.
    J Cell Sci 119(Pt 10) 1985-91(2006) 16621892
    Sato A, Toyoshima KE, Toki H, Ishibashi N, Asakawa K, Iwadate A, Kanayama T, Tobe H, Takeda A, Tsuji T.
    Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection.
    J Dermatol 39(8) 682-7(2012) 22380609
    Osada A, Kobayashi K, Masui S, Hamazaki TS, Yasuda K, Okochi H.
    Cloned cells from the murine dermal papilla have hair-inducing ability.
    J Dermatol Sci 54(2) 129-31(2009) 19150224
    Nishida T, Tsuji S, Tsujii M, Ishii S, Yoshio T, Shinzaki S, Egawa S, Irie T, Kakiuchi Y, Yasumaru M, Iijima H, Tsutsui S, Kawano S, Hayashi N.
    Cultured bone marrow cell local implantation accelerates healing of ulcers in mice.
    J Gastroenterol 43(2) 124-35(2008) 18306986
    Oura R, Arakaki R, Yamada A, Kudo Y, Tanaka E, Hayashi Y, Ishimaru N.
    Induction of rapid T cell death and phagocytic activity by Fas-deficient lpr macrophages.
    J Immunol 190(2) 578-85(2013) 23255359
    Kiguchi N, Kobayashi Y, Kadowaki Y, Fukazawa Y, Saika F, Kishioka S.
    Vascular endothelial growth factor signaling in injured nerves underlies peripheral sensitization in neuropathic pain.
    J Neurochem 129(1) 169-78(2014) 24304382
    Liu N, Deguchi K, Yamashita T, Liu W, Ikeda Y, Abe K.
    Intracerebral transplantation of bone marrow stromal cells ameliorates tissue plasminogen activator-induced brain damage after cerebral ischemia in mice detected by in vivo and ex vivo optical imaging.
    J Neurosci Res 90(11) 2086-93(2012) 22791305
    Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano HJ, Kawakami Y, Toyama Y, Toda M.
    Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery.
    J Neurosci Res 76(4) 453-65(2004) 15114617
    Ishigaki H, Yamamoto Y, Ishida H, Kajino K, Itoh Y, Fujiyama Y, Ogasawara K.
    Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
    Microbiol Immunol 50(8) 655-62(2006) 16924151
    Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T.
    Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ.
    Nat Commun 4 2497(2013) 24084941
    Toyoshima KE, Asakawa K, Ishibashi N, Toki H, Ogawa M, Hasegawa T, Irié T, Tachikawa T, Sato A, Takeda A, Tsuji T.
    Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches.
    Nat Commun 3 784(2012) 22510689
    Nakao K, Morita R, Saji Y, Ishida K, Tomita Y, Ogawa M, Saitoh M, Tomooka Y, Tsuji T.
    The development of a bioengineered organ germ method.
    Nat Methods 4(3) 227-30(2007) 17322892
    Ashina K, Tsubosaka Y, Nakamura T, Omori K, Kobayashi K, Hori M, Ozaki H, Murata T.
    Histamine Induces Vascular Hyperpermeability by Increasing Blood Flow and Endothelial Barrier Disruption In Vivo.
    PLoS One 10(7) e0132367(2015) 26158531
    Vogelgesang A, Scapin C, Barone C, Tam E, Blumental Perry A, Dammann CE.
    Cigarette smoke exposure during pregnancy alters fetomaternal cell trafficking leading to retention of microchimeric cells in the maternal lung.
    PLoS One 9(5) e88285(2014) 24832066
    Baba M, Kitoh K, Takashima Y.
    Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture.
    Parasitol Int 65(5 Pt B) 536-538(2016) 26806654
    Unno A, Suzuki K, Xuan X, Nishikawa Y, Kitoh K, Takashima Y.
    Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow.
    Parasitol Int 57(4) 515-8(2008) 18652914
    Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, Ogawa M, Mizuno M, Kasugai S, Tsuji T.
    Fully functional bioengineered tooth replacement as an organ replacement therapy.
    Proc Natl Acad Sci U S A 106(32) 13475-80(2009) 19666587
    Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.
    Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells.
    Proc Natl Acad Sci U S A 104(30) 12389-94(2007) 17636128
    González-González E, López-Casas PP, Del Mazo J.
    Gene silencing by RNAi in mouse Sertoli cells.
    Reprod Biol Endocrinol 6 29(2008) 18620581
    Asakawa K, Toyoshima KE, Ishibashi N, Tobe H, Iwadate A, Kanayama T, Hasegawa T, Nakao K, Toki H, Noguchi S, Ogawa M, Sato A, Tsuji T.
    Hair organ regeneration via the bioengineered hair follicular unit transplantation.
    Sci Rep 2 424(2012) 22645640
    Nakano-Doi A, Nakagomi T, Fujikawa M, Nakagomi N, Kubo S, Lu S, Yoshikawa H, Soma T, Taguchi A, Matsuyama T.
    Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction.
    Stem Cells 28(7) 1292-302(2010) 20517983