Strain Information | |
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Image | |
BRC No. | RBRC00235 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129X1-Chrng<tm1Mtak>/10Rbrc |
Former Common name | C57BL/6-TgH(AchR-gamma/10) |
H-2 Haplotype | |
ES Cell line | RW4 [129X1/SvJ] |
Background strain | C57BL/6JJcl |
Appearance | black |
Strain development | Developed by Dr. Masazumi Takahashi. This strain has been introduced into C57BL/6 genetic background by successive backcrossing for more than 10 generations in RIKEN Tsukuba Institute. |
Strain description | The acetylcholine receptors are known to have important roles in neuro-muscular functions. The gamma subunit are expressed in embryonic stages and the epsilon subunit are expressed postnatal stages.The acetylcholine receptors gamma and epsilon subunits have been disrupted by homologous recombination to study the functions of these genes and switching mechanism of gamma to epsilon subunits. The gamma subunit deficient homozygotes died after two days post delivery, suffering from inability of motor function of the limbs and drinking milk. The analysis on the neuro-muscular junctions showed the structural and electrophysiological abnormalities. |
Colony maintenance | Backcrossing to C57BL/6. Or sister-brother mating of heterozygotes. Breeding performance is good. |
References | Deficient development and maintenance of postsynaptic specializations in mutant mice lacking an 'adult' acetylcholine receptor subunit. Missias A C, Mudd J, Cunningham J M, Steinbach J H, Merlie J P, Sanes J R Development, 124, 5075-5086 (1997). 9362465Mitochondrial stress protein recognition of inactivated dehydrogenases during mammalian cell death. Bruschi S A, Lindsay J G, Crabb J W Proc. Natl. Acad. Sci. USA, 95, 13413-13418 (1998). 9811814Differential regulation of muscle acetylcholine receptor gamma- and epsilon-subunit mRNAs. Witzemann V, Barg B, Nishikawa Y, Sakmann B, Numa S FEBS Lett., 223, 104-112 (1987). 3666131Spontaneous muscle action potentials fail to develop without fetal-type acetylcholine receptors. Takahashi M, Kubo T, Mizoguchi A, Carlson C G, Endo K, Ohnishi K EMBO Rep., 3, 674-681 (2002). 12101101Acetylcholine receptor epsilon-subunit deletion causes muscle weakness and atrophy in juvenile and adult mice. Witzemann V, Schwarz H, Koenen M, Berberich C, Villarroel A, Wernig A, Brenner H R, Sakmann B Proc. Natl. Acad. Sci. USA, 93, 13286-13291 (1996). 8917583 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Chrng | cholinergic receptor, nicotinic, gamma polypeptide | 1 | Chrng | targeted mutation 1, Masazumi Takahashi | |||
neo | neomycin resistance gene (E. coli) | 1 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 8 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse muscle type nicotinic acetylcholine receptor gamma subunit |
Research application | Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masazumi Takahashi (Centre National de Genotypage) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Liu Y, Padgett D, Takahashi M, Li H, Sayeed A, Teichert RW, Olivera BM, McArdle JJ, Green WN, Lin W. Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning. Development 135(11) 1957-67(2008) 18434415 |