Strain Data Sheet

RBRC00234

Information1

Image
BRC No.RBRC00234
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Chrne<tm1Mtak>/Rbrc
Former Common nameC57BL/6-TgH(AchR-epsilon)
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack
Strain development
Strain description
Colony maintenance
ReferencesDevelopment, 124, 5075-5086 (1997). 9362465
Proc. Natl. Acad. Sci. USA, 95, 13413-13418 (1998). 9811814
FEBS Lett., 223, 104-112 (1987). 3666131
Proc. Natl. Acad. Sci. USA, 93, 13286-13291 (1996). 8917583
EMBO Rep., 3, 674-681 (2002). 12101101

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Chrnecholinergic receptor, nicotinic, epsilon polypeptide11Chrne<tm1Mtak>targeted mutation 1, Masazumi Takahashimouse muscle type nicotinic acetylcholine receptor epsilon subunit; Acre; AChrepsilon

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse muscle type nicotinic acetylcholine receptor epsilon subunit genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasazumi Takahashi (Centre National de Genotypage)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data