Strain Data Sheet


Strain Information

BRC No.RBRC00193
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameGRS.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameGRS/A-TgH(Tnc), GR-TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainGRS/A
Appearancealbino [a/a, c/c]
Strain developmentThe original tenascin deficient mice with mixed genetic background was reported to develop normally without tenascin. To clarify the biological function of tenascin-C in vivo, several congenic strains with inbred background have been developed by Dr. Kusakabe M, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992.The tenascin-deficient congenic strains were thus developed by successive backcrossing into A, BALB/cA, C57BL/6N, C3H/HeN, and GRS/A.
Strain descriptionThis strain is useful to study the biological function of extracellular matrix tenascin-C in vivo, espacially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. Background strain, GRS/A have a high incidence of mammary tumours which are highly hormone-responsive in breeding females.
Colony maintenance
ReferencesGene, 104, 177-185 (1991). 1717349
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782
Am. J. Pathol., 152, 1237-1245 (1998). 9588892
Genes Dev., 6, 1821-1831 (1992). 1383086
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
J. Neurosci., 15, 1971-1983 (1995). 7534342
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
J. Comp. Neurol., 430, 485-500 (2001). 11169482
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi AizawaTnc; tenascin C, Hxb, Ten, TN-C, AI528729

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999).
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data