Strain Data Sheet

RBRC00193

Information1

Image
BRC No.RBRC00193
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameGRS.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameGRS/A-TgH(Tnc), GR-TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainGRS/A
Appearancealbino [a/a, c/c]
Strain developmentThe original tenascin deficient mice with mixed genetic background was reported to develop normally without tenascin. To clarify the biological function of tenascin-C in vivo, several congenic strains with inbred background have been developed by Dr. Kusakabe M, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992.The tenascin-deficient congenic strains were thus developed by successive backcrossing into A, BALB/cA, C57BL/6N, C3H/HeN, and GRS/A.
Strain descriptionThis strain is useful to study the biological function of extracellular matrix tenascin-C in vivo, espacially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. Background strain, GRS/A have a high incidence of mammary tumours which are highly hormone-responsive in breeding females.
Colony maintenance
ReferencesAm. J. Pathol., 152, 1237-1245 (1998). 9588892
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
J. Comp. Neurol., 430, 485-500 (2001). 11169482
Genes Dev., 6, 1821-1831 (1992). 1383086
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Gene, 104, 177-185 (1991). 1717349
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993
J. Neurosci., 15, 1971-1983 (1995). 7534342

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi AizawaTnc; tenascin C, Hxb, Ten, TN-C, AI528729

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tenascin genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999).
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data