Strain Data Sheet

RBRC00169

Information1

Image
BRC No.RBRC00169
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameC57BL/6N-TgH(Tnc), B6-TN, TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NJcl
Appearanceblack [a/a, B/B, C/C]
Strain developmentCongenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N.
Strain descriptionTenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice are viable and fertile.
ReferencesAm. J. Pathol., 152, 1237-1245 (1998). 9588892
Genes Dev., 6, 1821-1831 (1992). 1383086
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993
Jpn. J. Cancer Res., 93, 968-975 (2002). 12359049
J. Neurosci., 15, 1971-1983 (1995). 7534342
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782
J. Comp. Neurol., 430, 485-500 (2001). 11169482
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540
Gene, 104, 177-185 (1991). 1717349

Health Report

Examination Date / Room / Rack2022/04/25Room:3-CRack:K
2022/02/14Room:3-CRack:K
2021/11/15Room:3-CRack:K
2021/08/16Room:3-CRack:K
2021/07/18Room:3-CRack:K
2021/05/17Room:3-CRack:K
2021/05/14Room:3-CRack:K
2021/02/15Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnctenascin C4Tnc<tm1Sia>targeted mutation 1, Shinichi AizawaTnc; tenascin C, Hxb, Ten, TN-C, AI528729

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999).
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y.
Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
Blood 119(23) 5429-37(2012) 22553313

Xu M, Ye Z, Zhao X, Guo H, Gong X, Huang R.
Deficiency of tenascin-C attenuated cardiac injury by inactivating TLR4/NLRP3/caspase-1 pathway after myocardial infarction.
Cell Signal 86 110084(2021) 34271086

Imanaka-Yoshida K, Matsumoto K, Hara M, Sakakura T, Yoshida T.
The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse.
Differentiation 71(4-5) 291-8(2003) 12823230

Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H.
Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C.
Jpn J Cancer Res 93(9) 968-75(2002) 12359049

Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T.
Integrin α9 on lymphatic endothelial cells regulates lymphocyte egress.
Proc Natl Acad Sci U S A 111(8) 3080-5(2014) 24516133

Kubo Y, Ishikawa K, Mori K, Kobayashi Y, Nakama T, Arima M, Nakao S, Hisatomi T, Haruta M, Sonoda KH, Yoshida S.
Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy.
Sci Rep 10(1) 9299(2020) 32518264