Strain Information | |
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Image | |
BRC No. | RBRC00169 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tnc<tm1Sia>/Rbrc |
Former Common name | C57BL/6N-TgH(Tnc), B6-TN, TNKO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6NJcl |
Appearance | black [a/a, B/B, C/C] |
Strain development | Congenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N. |
Strain description | Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193). |
Colony maintenance | Homozygote x HomozygoteHomozygous mutant mice are viable and fertile. |
References | Abnormal behavior and neurotransmissions of tenascin gene knockout mouse. Fukamauchi F, Mataga N, Wang Y J, Sato S, Youshiki A, Kusakabe M Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse. Koyama Y, Kusubata M, Yoshiki A, Hiraiwa N, Ohashi T, Irie S, Kusakabe M J. Invest. Dermatol., 111, 930-935 (1998). 9856798Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C. Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H Jpn. J. Cancer Res., 93, 968-975 (2002). 12359049Tenascin-C expression and splice variant in habu snake venom-induced glomerulonephritis. Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M Exp. Mol. Pathol., 72, 186-195 (2002). 12009782Tyrosine hydroxylase activity and its mRNA level in dopaminergic neurons of tenascin gene knockout mouse. Fukamauchi F, Mataga N, Wang Y J, Sato S, Yoshiki A, Kusakabe M Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278Murine tenascin: cDNA cloning, structure and temporal expression of isoforms. Saga Y, Tsukamoto T, Jing N, Kusakabe M, Sakakura T Gene, 104, 177-185 (1991). 1717349PDGF receptor-alpha deficiency in glomerular mesangial cells of tenascin-C knockout mice. Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993Corneal wound healing in tenascin knockout mouse. Matsuda A, Yoshiki A, Tagawa Y, Matsuda H, Kusakabe M Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540Can tenascin be redundant in cancer development? Sakakura T, Kusakabe M Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137Mice develop normally without tenascin. Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S Genes Dev., 6, 1821-1831 (1992). 1383086more 3 referencesTenascin-C promotes healing of Habu-snake venom-induced glomerulonephritis: studies in knockout congenic mice and in culture. Nakao N, Hiraiwa N, Yoshiki A, Ike F, Kusakabe M Am. J. Pathol., 152, 1237-1245 (1998). 9588892Loss of cortical and thalamic neuronal tenascin-C expression in a transgenic mouse expressing exon 1 of the human Huntington disease gene. Kusakabe M, Mangiarini L, Laywell E D, Bates G P, Yoshiki A, Hiraiwa N, Inoue J, Steindler D A J. Comp. Neurol., 430, 485-500 (2001). 11169482Tenascin knockout mice: barrels, boundary molecules, and glial scars. Steindler D A, Settles D, Erickson H P, Laywell E D, Yoshiki A, Faissner A, Kusakabe M J. Neurosci., 15, 1971-1983 (1995). 7534342 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Tnc | tenascin C | 4 | Tnc | targeted mutation 1, Shinichi Aizawa | |||
lacZ | beta-galactosidase (E. coli) | 4 | |||||
neo | neomycin resistance gene (E. coli) | 4 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 3 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA |
Research application | Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999). |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood 119(23) 5429-37(2012) 22553313 |
Yonemura A, Semba T, Zhang J, Fan Y, Yasuda-Yoshihara N, Wang H, Uchihara T, Yasuda T, Nishimura A, Fu L, Hu X, Wei F, Kitamura F, Akiyama T, Yamashita K, Eto K, Iwagami S, Iwatsuki M, Miyamoto Y, Matsusaki K, Yamasaki J, Nagano O, Saya H, Song S, Tan P, Baba H, Ajani JA, Ishimoto T. Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment. Cell Rep 43(1) 113613(2024) 38232734 |
Xu M, Ye Z, Zhao X, Guo H, Gong X, Huang R. Deficiency of tenascin-C attenuated cardiac injury by inactivating TLR4/NLRP3/caspase-1 pathway after myocardial infarction. Cell Signal 86 110084(2021) 34271086 |
Imanaka-Yoshida K, Matsumoto K, Hara M, Sakakura T, Yoshida T. The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse. Differentiation 71(4-5) 291-8(2003) 12823230 |
Tashjian RZ, Zitnay J, Kazmers NH, Veerabhadraiah SR, Zelada AC, Honeggar M, Smith MC, Chalmers PN, Henninger HB, Jurynec MJ. Tenascin C deletion impairs tendon healing and functional recovery after rotator cuff repair. J Orthop Res (2024) 39601211 |
Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H. Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C. Jpn J Cancer Res 93(9) 968-75(2002) 12359049 |
Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T. Integrin α9 on lymphatic endothelial cells regulates lymphocyte egress. Proc Natl Acad Sci U S A 111(8) 3080-5(2014) 24516133 |
Kubo Y, Ishikawa K, Mori K, Kobayashi Y, Nakama T, Arima M, Nakao S, Hisatomi T, Haruta M, Sonoda KH, Yoshida S. Periostin and tenascin-C interaction promotes angiogenesis in ischemic proliferative retinopathy. Sci Rep 10(1) 9299(2020) 32518264 |