Strain Data Sheet

RBRC00164

Strain Information

Image
BRC No.RBRC00164
TypeSpontaneous Mutation Congenic
SpeciesMus musculus
Strain nameB6.C3-Kit<W-f>/Rbrc
Former Common nameC57BL/6-W<f>/+
H-2 Haplotype
ES Cell line
Background strain
AppearanceBlack with extensive depigmented areas lacking a defined pattern [a/a B/B C/C W/W]
Strain developmentSpontaneous mutation originally arosed in C3H/HePas. The B6.C3-Kit<W-f> congenic strain was generated by backcrossed to C57BL/6 by Dr. Y. Kitamura, then deposited to the RIKEN BRC.
Strain descriptionB6.C3-Kit<W-f>/Rbrc. Fertile dominant spotting. The original Kit<W-f> mutation arose spontaneously in the C3H/HePas strain. The B6.C3-Kit<W-f> congenic strain was generated by backcrossed to C57BL/6 by Dr. Y. Kitamura. Homozygous mice for Kit<W-f> are black with white sash. Typical dilution of pigmented areas not observed. The c-Kit cDNA from Kit<W-f>/ Kit<W-f> mice showed an AT to TA transversion at nucleotide 2474 that resulted in the change from Arg to Trp at position 816.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kit
MGI:96677		KIT proto-oncogene receptor tyrosine kinase5Kit<W-f>
MGI:1856261		fertile dominant spotting
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • belly spot(MP:0000373)

  • decreased erythrocyte cell number(MP:0002875)

  • decreased mast cell number(MP:0000336)

  • diluted coat color(MP:0000371)

  • head spot(MP:0002939)
    • more 5 phenotypes
  • irregular coat pigmentation(MP:0000372)

  • macrocytic anemia(MP:0002811)

  • non-pigmented tail tip(MP:0005175)

  • postnatal lethality(MP:0002082)

  • white spotting(MP:0002938)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorYukihiko Kitamura (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    BRC mice in Publications

    No Data