Strain Data Sheet

RBRC00159

Information1

Image
BRC No.RBRC00159
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Trp53<tm1Sia> Tg(gpt delta)1Nmi/Rbrc
Former Common nameC57BL/6-TgH(p53)-TgN(gpt delta), C57BL/6-p53 <tm1Sia>-Tg(gpt Delta)
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain developmentThe original p53 deficient mice had been introduced into C57BL/6 background for 13 generations by Dr. Niwa T at Hiroshima Univ (-1995). Then the mice were transfered to Dr. Kusakabe M. at RIKEN Life Science Center for further backcrossing to establish the B6 congenic strain. The gpt delta mice were crossed with C57BL/6-p53tm1 to establish a double mutant at RIKEN, and have been maintained by sister-brother mating for 8 generations.
Strain description
Colony maintenance
ReferencesCancer Lett., 143, 241-244 (1999). 10503911
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717
Carcinogenesis, 21, 2049-2056 (2000). 11062167
Cancer Res., 61, 3913-3918 (2001). 11358805
Phys. Med. 17 Suppl. 1:192-193, (2001). 11776258
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079
Oncogene, 8, 3313-3322 (1993). 7504233
Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728
Environ. Mutagen Res., 22, 85-90 (2000).

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation-related protein 5311Trp53<tm1Sia>targeted mutation 1, Shinichi Aizawap53

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
gpt Deltaguanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>transgene insertion 1, Takehito Nohmi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
red/gamlambda phage red/gam17red/gam

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA, lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
Research applicationApoptosis Research
Cancer Research
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
Toxicology Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Sakai H, Tsukamoto T, Yamamoto M, Shirai N, Iidaka T, Hirata A, Yanai T, Masegi T, Donehower LA, Tatematsu M.
High susceptibility of nullizygous p53 knockout mice to colorectal tumor induction by 1,2-dimethylhydrazine.
J Cancer Res Clin Oncol 129(6) 335-40(2003) 12743811

Masumura K, Horiguchi M, Nishikawa A, Umemura T, Kanki K, Kanke Y, Nohmi T.
Low dose genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in gpt delta transgenic mice.
Mutat Res 541(1-2) 91-102(2003) 14568298

Nishikawa A, Imazawa T, Kuroiwa Y, Kitamura Y, Kanki K, Ishii Y, Umemura T, Hirose M.
Induction of colon tumors in C57BL/6J mice fed MeIQx, IQ, or PhIP followed by dextran sulfate sodium treatment.
Toxicol Sci 84(2) 243-8(2005) 15635146