Strain Data Sheet

RBRC00158

Strain Information

Image
BRC No.RBRC00158
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(gpt delta)1Nmi/Rbrc
Former Common nameC57BL/6-TgN(gpt Delta)
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain development国立食品衛生安全性研究所の能美博士により生体を用いた突然変異検出系として開発。
Strain description点突然変異と欠失突然変異の検出および解析のために開発されたトランスジェニマウス
Colony maintenance兄妹交配　ホモ型トランスジェニックとして固定。
References
A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.
T Nohmi, M Katoh, H Suzuki, M Matsui, M Yamada, M Watanabe, M Suzuki, N Horiya, O Ueda, T Shibuya, H Ikeda, T Sofuni
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079

Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse.
K Masumura, K Matsui, M Yamada, M Horiguchi, K Ishida, M Watanabe, O Ueda, H Suzuki, Y Kanke, K R Tindall, K Wakabayashi, T Sofuni, T Nohmi
Cancer Lett., 143, 241-244 (1999). 10503911

UVB-induced gpt mutations in the skin of gpt delta transgenic mice.
M Horiguchi, K Masumura, H Ikehata, T Ono, Y Kanke, T Sofuni, T Nohmi
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728

Spectra of gpt mutations in ethylnitrosourea-treated and untreated transgenic mice.
K Masumura, M Matsui, M Katoh, N Horiya, O Ueda, H Tanabe, M Yamada, H Suzuki, T Sofuni, T Nohmi
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717

Spi(-) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice.
T Nohmi, M Suzuki, K Masumura, M Yamada, K Matsui, O Ueda, H Suzuki, M Katoh, H Ikeda, T Sofuni
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
O Minowa, T Arai, M Hirano, Y Monden, S Nakai, M Fukuda, M Itoh, H Takano, Y Hippou, H Aburatani, K Masumura, T Nohmi, S Nishimura, T Noda
Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358

Characterization of mutations induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in the colon of gpt delta transgenic mouse: novel G:C deletions beside runs of identical bases.
K Masumura, K Matsui, M Yamada, M Horiguchi, K Ishida, M Watanabe, K Wakabayashi, T Nohmi
Carcinogenesis, 21, 2049-2056 (2000). 11062167

Environ. Mutagen Res., 22, 85-90 (2000).

Molecular nature of ultraviolet B light-induced deletions in the murine epidermis.
M Horiguchi, K I Masumura, H Ikehata, T Ono, Y Kanke, T Nohmi
Cancer Res., 61, 3913-3918 (2001). 11358805

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cat E. coli chloramphenicol acetyltransferase (CAT)17
gpt Delta guanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>
MGI:3612991		transgene insertion 1, Takehito Nohmi
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
red/gam lambda phage red/gam17red/gam

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cell physiology (MP:0005621)
    • Detailed phenotype data

    Ordering Information

    供与核酸lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
    Research application
    提供条件条件を付加しない。
    Depositor能美　健彦（国立医薬品食品衛生研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data