Strain Information | |
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Image | |
BRC No. | RBRC00158 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(gpt delta)1Nmi/Rbrc |
Former Common name | C57BL/6-TgN(gpt Delta) |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6 |
Appearance | black [a/a] |
Strain development | This strain was developed for efficient detection of mutations in vivo by Dr.Nohmi T. |
Strain description | This strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli. |
Colony maintenance | |
References | Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice. Minowa O, Arai T, Hirano M, Monden Y, Nakai S, Fukuda M, Itoh M, Takano H, Hippou Y, Aburatani H, Masumura K, Nohmi T, Nishimura S, Noda T Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358Characterization of mutations induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in the colon of gpt delta transgenic mouse: novel G:C deletions beside runs of identical bases. Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Wakabayashi K, Nohmi T Carcinogenesis, 21, 2049-2056 (2000). 11062167UVB-induced gpt mutations in the skin of gpt delta transgenic mice. Horiguchi M, Masumura K, Ikehata H, Ono T, Kanke Y, Sofuni T, Nohmi T Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728Spectra of gpt mutations in ethylnitrosourea-treated and untreated transgenic mice. Masumura K, Matsui M, Katoh M, Horiya N, Ueda O, Tanabe H, Yamada M, Suzuki H, Sofuni T, Nohmi T Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse. Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Ueda O, Suzuki H, Kanke Y, Tindall K R, Wakabayashi K, Sofuni T, Nohmi T Cancer Lett., 143, 241-244 (1999). 10503911Molecular nature of ultraviolet B light-induced deletions in the murine epidermis. Horiguchi M, Masumura K I, Ikehata H, Ono T, Kanke Y, Nohmi T Cancer Res., 61, 3913-3918 (2001). 11358805Spi(-) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice. Nohmi T, Suzuki M, Masumura K, Yamada M, Matsui K, Ueda O, Suzuki H, Katoh M, Ikeda H, Sofuni T Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections. Nohmi T, Katoh M, Suzuki H, Matsui M, Yamada M, Watanabe M, Suzuki M, Horiya N, Ueda O, Shibuya T, Ikeda H, Sofuni T Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079Environ. Mutagen Res., 22, 85-90 (2000). |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
cat | E. coli chloramphenicol acetyltransferase (CAT) | 17 | |||||
gpt Delta | guanine phosphoribosyltransferase | 17 | gpt Delta | transgene insertion 1, Takehito Nohmi | |||
loxP | phage P1 loxP | 17 | loxP | ||||
loxP | phage P1 loxP | 17 | loxP | ||||
red/gam | lambda phage red/gam | 17 | red/gam |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC |
Research application | |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Takehiko Noumi (National Institute of Health Sciences) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |