Strain Information | |
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Image | |
BRC No. | RBRC00109 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc |
Former Common name | C3H/HeN-TgH(Zic1), Zic1 KO (C3H background) |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae](D3 subline) |
Background strain | C3H/HeNJcl |
Appearance | agouti |
Strain development | Developed by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations. |
Strain description | The Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.Genotype of Affected Mutation:Zic1 null heterozygous. |
Colony maintenance | Backcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs. |
References | Mol. Brain Res., 78, 15-25 (2000). 10891581 Mech. Dev., 89, 141-150 (1999). 10559489 Dev. Biol., 244, 329-341 (2002). 11944941 J. Neurosci., 18, 284-293 (1998). 9412507 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Zic1zinc finger protein of the cerebellum 19Zic1<tm1Jaru>targeted mutation 1, Jun Arugazinc finger protein of the cerebellum 1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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供与核酸 | mouse Zic1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) |
Research application | Developmental Biology Research Mouse Models for Human Disease Neurobiology Research |
提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 18, 284-293 (1998). 研究成果の公表にあたって謝辞の表明を必要とする。 |
Depositor | 有賀 純(理化学研究所脳科学総合研究センター) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |