Strain Data Sheet


Strain Information

BRC No.RBRC00109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc
Former Common nameC3H/HeN-TgH(Zic1), Zic1 KO (C3H background)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae](D3 subline)
Background strainC3H/HeNJcl
Strain developmentDeveloped by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations.
Strain descriptionThe Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.Genotype of Affected Mutation:Zic1 null heterozygous.
Colony maintenanceBackcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs.
ReferencesMech. Dev., 89, 141-150 (1999). 10559489
J. Neurosci., 18, 284-293 (1998). 9412507
Dev. Biol., 244, 329-341 (2002). 11944941
Mol. Brain Res., 78, 15-25 (2000). 10891581

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zic1zinc finger protein of the cerebellum 19Zic1<tm1Jaru>targeted mutation 1, Jun Arugazinc finger protein of the cerebellum 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse Zic1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationDevelopmental Biology Research
Mouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 18, 284-293 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorJun Aruga (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data