Strain Data Sheet

RBRC00109

Strain Information

Image
BRC No.RBRC00109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc
Former Common nameC3H/HeN-TgH(Zic1), Zic1 KO (C3H background)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae](D3 subline)
Background strainC3H/HeNJcl
Appearanceagouti
Strain developmentDeveloped by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations.
Strain descriptionThe Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.Genotype of Affected Mutation:Zic1 null heterozygous.
Colony maintenanceBackcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs.
References
Mouse Zic1 is involved in cerebellar development.
Aruga J, Minowa O, Yaginuma H, Kuno J, Nagai T, Noda T, Mikoshiba K
J. Neurosci., 18, 284-293 (1998). 9412507

Zic1 regulates the patterning of vertebral arches in cooperation with Gli3.
Aruga J, Mizugishi K, Koseki H, Imai K, Balling R, Noda T, Mikoshiba K
Mech. Dev., 89, 141-150 (1999). 10559489

A 5' segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
Aruga J, Shimoda K, Mikoshiba K
Mol. Brain Res., 78, 15-25 (2000). 10891581

Zic1 promotes the expansion of dorsal neural progenitors in spinal cord by inhibiting neuronal differentiation.
Aruga J, Tohmonda T, Homma S, Mikoshiba K
Dev. Biol., 244, 329-341 (2002). 11944941

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zic1
MGI:106683		zinc finger protein of the cerebellum 19Zic1<tm1Jaru>
MGI:2156822		targeted mutation 1, Jun Aruga
  • craniosynostosis 6(MedGEN)

  • structural brain anomalies with impaired intellectual development and craniosynostosis(MedGEN)
    • neo neomycin resistance gene (E. coli)9 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cerebellar foliation(MP:0000857)

  • abnormal cerebellum development(MP:0000854)

  • abnormal cerebellum vermis morphology(MP:0000864)

  • abnormal gait(MP:0001406)

  • abnormal suckling behavior(MP:0001436)
    • more 12 phenotypes
  • ataxia(MP:0001393)

  • cerebellum hypoplasia(MP:0000851)

  • decreased locomotor activity(MP:0001402)

  • hypotonia(MP:0004144)

  • impaired limb coordination(MP:0001524)

  • impaired righting response(MP:0001523)

  • kyphosis(MP:0000160)

  • neonatal lethality, incomplete penetrance(MP:0011088)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, complete penetrance(MP:0011085)

  • premature death(MP:0002083)

  • reduced cerebellar foliation(MP:0009719)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Zic1 genomic DNA
    Research applicationDevelopmental Biology Research
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 18, 284-293 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorJun Aruga (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data