Strain Data Sheet

RBRC00107

Strain Information

Image
BRC No.RBRC00107
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC3.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameC3H/HeN-TgH(p53)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC3H/HeNCrlCrlj
Appearanceagouti
Strain development理化学研究所筑波研究所・相沢慎一。TT2 ES細胞（B6CBAF1由来）を用いて作出。京都大学放射線生物研究センター・丹羽大貫（C57BL/6背景、ICR背景およびC3H背景）、大阪大学大学院生命機能研究科・八木　健（DBA/2背景）、新潟大学大学院医歯学総合研究科・木南　凌（MSM背景）らによりコンジェニック育成。
Strain descriptionp53 (Trp53) 遺伝子のノックアウトマウス。p53はアポトーシス、細胞周期、DNA修復機構に関与するガン抑制遺伝子。第2エクソンにhomologous recombinationによりneo遺伝子を挿入。ホモマウスは様々な部位でガンが発生する (3から6ヶ月齢頃までに) 。C57BL/6背景 (RBRC01361) 、ICR背景 (RBRC01348) 、C3H背景 (RBRC00107) 、DBA/2背景 (RBRC01518) およびMSM背景 (RBRC00815) があり。ガンを発症する前であればホモマウスも繁殖可能 (成績は低い) 。C57BL/6背景ではホモマウスのメスはほとんど生まれてこない (MSM背景もおそらく同様) 。
Colony maintenanceHeterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj]
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l
Oncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<tm1Sia>
MGI:1926340		targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 20 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast cancer(DisGeNET)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(DisGeNET, MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • lung cancer(DisGeNET)

  • lung neoplasm(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)

  • osteosarcoma(DisGeNET)

  • pancreatic neoplasm(DisGeNET)

  • sarcoma(DisGeNET)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cell proliferation (MP:0000350)

  • abnormal lymphatic vessel morphology (MP:0001879)

  • decreased cellular sensitivity to ultraviolet irradiation (MP:0008411)

  • embryonic growth retardation (MP:0003984)

  • increased T cell derived lymphoma incidence (MP:0002024)
    • more 9 phenotypes
  • increased apoptosis (MP:0006042)

  • increased hemangiosarcoma incidence (MP:0003667)

  • increased lymphoma incidence (MP:0012431)

  • increased rhabdomyosarcoma incidence (MP:0002036)

  • increased sensitivity to induced cell death (MP:0008943)

  • increased teratoma incidence (MP:0002627)

  • increased tumor incidence (MP:0002020)

  • myositis (MP:0004510)

  • premature death (MP:0002083)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    提供条件条件を付加しない。
    Depositor丹羽　太貫（京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005

    BRC mice in Publications

    Machida Y, Imai T.
    Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice.
    Oncol Lett 22(4) 738(2021) 34466150
    Naruse M, Masui R, Ochiai M, Maru Y, Hippo Y, Imai T.
    An organoid-based carcinogenesis model induced by in vitro chemical treatment.
    Carcinogenesis 41(10) 1444-1453(2020) 32047892
    Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
    Activation of interferon-stimulated genes by gamma-ray irradiation independently of the ataxia telangiectasia mutated-p53 pathway.
    Mol Cancer Res 9(4) 476-84(2011) 21357441
    Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
    p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation.
    Radiat Res 175(3) 328-35(2011) 21388276
    Sugihara T, Murano H, Tanaka K, Oghiso Y.
    Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells.
    J Radiat Res 49(3) 231-40(2008) 18285661