Strain Information | |
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Image | |
BRC No. | RBRC00107 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | C3.Cg-Trp53<tm1Sia>/Rbrc |
Former Common name | C3H/HeN-TgH(p53) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C3H/HeNCrlCrlj |
Appearance | agouti |
Strain development | Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences. |
Strain description | Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background). |
Colony maintenance | Heterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj] |
References | Enhanced proliferative potential in culture of cells from p53-deficient mice. Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l Oncogene, 8, 3313-3322 (1993). 7504233 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Trp53 | transformation related protein 53 | 11 | Trp53 | targeted mutation 1, Shinichi Aizawa | more 14 Diseases | ||
neo | neomycin resistance gene (E. coli) | 11 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | more 14 Diseases |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 9 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA |
Research application | Cancer Research Cell Biology Research Immunology and Inflammation Research Mouse Models for Human Disease |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Ohtsura Niwa (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2005 |
BRC mice in Publications |
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Naruse M, Masui R, Ochiai M, Maru Y, Hippo Y, Imai T. An organoid-based carcinogenesis model induced by in vitro chemical treatment. Carcinogenesis 41(10) 1444-1453(2020) 32047892 |
Sugihara T, Murano H, Tanaka K, Oghiso Y. Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells. J Radiat Res 49(3) 231-40(2008) 18285661 |
Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K. Activation of interferon-stimulated genes by gamma-ray irradiation independently of the ataxia telangiectasia mutated-p53 pathway. Mol Cancer Res 9(4) 476-84(2011) 21357441 |
Machida Y, Imai T. Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice. Oncol Lett 22(4) 738(2021) 34466150 |
Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K. p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation. Radiat Res 175(3) 328-35(2011) 21388276 |