Strain Data Sheet

RBRC00107

Strain Information

Image
BRC No.RBRC00107
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC3.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameC3H/HeN-TgH(p53)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC3H/HeNCrlCrlj
Appearanceagouti
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj]
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l
Oncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53transformation related protein 5311Trp53targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
  • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
  • neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
  • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell proliferation(MP:0000350)

  • abnormal lymphatic vessel morphology(MP:0001879)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • embryonic growth retardation(MP:0003984)

  • increased T cell derived lymphoma incidence(MP:0002024)
  • more 9 phenotypes
  • increased apoptosis(MP:0006042)

  • increased hemangiosarcoma incidence(MP:0003667)

  • increased lymphoma incidence(MP:0012431)

  • increased rhabdomyosarcoma incidence(MP:0002036)

  • increased sensitivity to induced cell death(MP:0008943)

  • increased teratoma incidence(MP:0002627)

  • increased tumor incidence(MP:0002020)

  • myositis(MP:0004510)

  • premature death(MP:0002083)
  • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorOhtsura Niwa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005

    BRC mice in Publications

    Naruse M, Masui R, Ochiai M, Maru Y, Hippo Y, Imai T.
    An organoid-based carcinogenesis model induced by in vitro chemical treatment.
    Carcinogenesis 41(10) 1444-1453(2020) 32047892
    Sugihara T, Murano H, Tanaka K, Oghiso Y.
    Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells.
    J Radiat Res 49(3) 231-40(2008) 18285661
    Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
    Activation of interferon-stimulated genes by gamma-ray irradiation independently of the ataxia telangiectasia mutated-p53 pathway.
    Mol Cancer Res 9(4) 476-84(2011) 21357441
    Machida Y, Imai T.
    Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice.
    Oncol Lett 22(4) 738(2021) 34466150
    Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
    p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation.
    Radiat Res 175(3) 328-35(2011) 21388276