Strain Data Sheet

RBRC00067

Strain Information

Image
BRC No.RBRC00067
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Tnc<tm1Sia>/Rbrc
Former Common nameBALB/cA-TgH(Tnc), BALB/c-TN, TNKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAJcl
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. M. Kusakabe. Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN in 1990's. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to BALB/cA.
Strain descriptionC.B6CB-Tnc<tm1Sia>. Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the feild of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction, etc. The tenascin C deficient congenic strains were developed successive by backcrossing into A (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenanceHomozygote x HomozygoteHomozygous mutant mice are viable and fertile.
References
Tenascin knockout mice: barrels, boundary molecules, and glial scars.
Steindler D A, Settles D, Erickson H P, Laywell E D, Yoshiki A, Faissner A, Kusakabe M
J. Neurosci., 15, 1971-1983 (1995). 7534342

PDGF receptor-alpha deficiency in glomerular mesangial cells of tenascin-C knockout mice.
Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M
Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993

Mice develop normally without tenascin.
Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S
Genes Dev., 6, 1821-1831 (1992). 1383086

Tenascin-C promotes healing of Habu-snake venom-induced glomerulonephritis: studies in knockout congenic mice and in culture.
Nakao N, Hiraiwa N, Yoshiki A, Ike F, Kusakabe M
Am. J. Pathol., 152, 1237-1245 (1998). 9588892

Tenascin-C expression and splice variant in habu snake venom-induced glomerulonephritis.
Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M
Exp. Mol. Pathol., 72, 186-195 (2002). 12009782

Can tenascin be redundant in cancer development?
Sakakura T, Kusakabe M
Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137

Abnormal behavior and neurotransmissions of tenascin gene knockout mouse.
Fukamauchi F, Mataga N, Wang Y J, Sato S, Youshiki A, Kusakabe M
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327

Loss of cortical and thalamic neuronal tenascin-C expression in a transgenic mouse expressing exon 1 of the human Huntington disease gene.
Kusakabe M, Mangiarini L, Laywell E D, Bates G P, Yoshiki A, Hiraiwa N, Inoue J, Steindler D A
J. Comp. Neurol., 430, 485-500 (2001). 11169482

Corneal wound healing in tenascin knockout mouse.
Matsuda A, Yoshiki A, Tagawa Y, Matsuda H, Kusakabe M
Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540

Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse.
Koyama Y, Kusubata M, Yoshiki A, Hiraiwa N, Ohashi T, Irie S, Kusakabe M
J. Invest. Dermatol., 111, 930-935 (1998). 9856798
more 5 references
Murine tenascin: cDNA cloning, structure and temporal expression of isoforms.
Saga Y, Tsukamoto T, Jing N, Kusakabe M, Sakakura T
Gene, 104, 177-185 (1991). 1717349

Tyrosine hydroxylase activity and its mRNA level in dopaminergic neurons of tenascin gene knockout mouse.
Fukamauchi F, Mataga N, Wang Y J, Sato S, Yoshiki A, Kusakabe M
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278

乳癌基礎研究, 8, 26-31 (1999).

乳癌基礎研究, 5, 41-45 (1996).

炎症, 15(5), 377-381 (1996).

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnctenascin C4Tnctargeted mutation 1, Shinichi Aizawa
  • autosomal dominant nonsyndromic hearing loss 56(MedGEN)
  • lacZbeta-galactosidase (E. coli)4
  • autosomal dominant nonsyndromic hearing loss 56(MedGEN)
  • neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal dominant nonsyndromic hearing loss 56(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal CNS synaptic transmission(MP:0002206)

  • abnormal circadian behavior(MP:0020467)

  • abnormal serotonin level(MP:0005322)

  • decreased dopamine level(MP:0005643)

  • hearing/vestibular/ear phenotype(MP:0005377)
  • more 3 phenotypes
  • hyperactivity(MP:0001399)

  • impaired swimming(MP:0001522)

  • increased stereotypic behavior(MP:0001409)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Vis. Sci., 40, 1071-1080 (1999).
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Kubo Y, Arima M, Nakao S, Hisatomi T, Ikeda Y, Matsuda A, Sonoda KH, Ishibashi T.
    Tenascin-C secreted by transdifferentiated retinal pigment epithelial cells promotes choroidal neovascularization via integrin αV.
    Lab Invest 96(11) 1178-1188(2016) 27668890