Strain Information | |
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Image | |
BRC No. | RBRC00067 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | C.Cg-Tnc<tm1Sia>/Rbrc |
Former Common name | BALB/cA-TgH(Tnc), BALB/c-TN, TNKO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | BALB/cAJcl |
Appearance | albino [A/A b/b c/c] |
Strain development | Developed by Dr. M. Kusakabe. Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN in 1990's. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to BALB/cA. |
Strain description | C.B6CB-Tnc<tm1Sia>. Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the feild of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction, etc. The tenascin C deficient congenic strains were developed successive by backcrossing into A (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193). |
Colony maintenance | Homozygote x HomozygoteHomozygous mutant mice are viable and fertile. |
References | Tenascin knockout mice: barrels, boundary molecules, and glial scars. Steindler D A, Settles D, Erickson H P, Laywell E D, Yoshiki A, Faissner A, Kusakabe M J. Neurosci., 15, 1971-1983 (1995). 7534342PDGF receptor-alpha deficiency in glomerular mesangial cells of tenascin-C knockout mice. Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M Biochem. Biophys. Res. Commun., 290, 1220-1227 (2002). 11811993Mice develop normally without tenascin. Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S Genes Dev., 6, 1821-1831 (1992). 1383086Tenascin-C promotes healing of Habu-snake venom-induced glomerulonephritis: studies in knockout congenic mice and in culture. Nakao N, Hiraiwa N, Yoshiki A, Ike F, Kusakabe M Am. J. Pathol., 152, 1237-1245 (1998). 9588892Tenascin-C expression and splice variant in habu snake venom-induced glomerulonephritis. Matsumoto K, Hiraiwa N, Yoshiki A, Ohnishi M, Kusakabe M Exp. Mol. Pathol., 72, 186-195 (2002). 12009782Can tenascin be redundant in cancer development? Sakakura T, Kusakabe M Perspect. Dev. Neurobiol., 2, 111-116 (1994). 7530137Abnormal behavior and neurotransmissions of tenascin gene knockout mouse. Fukamauchi F, Mataga N, Wang Y J, Sato S, Youshiki A, Kusakabe M Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327Loss of cortical and thalamic neuronal tenascin-C expression in a transgenic mouse expressing exon 1 of the human Huntington disease gene. Kusakabe M, Mangiarini L, Laywell E D, Bates G P, Yoshiki A, Hiraiwa N, Inoue J, Steindler D A J. Comp. Neurol., 430, 485-500 (2001). 11169482Corneal wound healing in tenascin knockout mouse. Matsuda A, Yoshiki A, Tagawa Y, Matsuda H, Kusakabe M Invest. Ophthalmol. Visual. Sci., 40, 1071-1080 (1999). 10235540Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse. Koyama Y, Kusubata M, Yoshiki A, Hiraiwa N, Ohashi T, Irie S, Kusakabe M J. Invest. Dermatol., 111, 930-935 (1998). 9856798more 5 referencesMurine tenascin: cDNA cloning, structure and temporal expression of isoforms. Saga Y, Tsukamoto T, Jing N, Kusakabe M, Sakakura T Gene, 104, 177-185 (1991). 1717349Tyrosine hydroxylase activity and its mRNA level in dopaminergic neurons of tenascin gene knockout mouse. Fukamauchi F, Mataga N, Wang Y J, Sato S, Yoshiki A, Kusakabe M Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278乳癌基礎研究, 8, 26-31 (1999). 乳癌基礎研究, 5, 41-45 (1996). 炎症, 15(5), 377-381 (1996). |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Tnc | tenascin C | 4 | Tnc | targeted mutation 1, Shinichi Aizawa | |||
lacZ | beta-galactosidase (E. coli) | 4 | |||||
neo | neomycin resistance gene (E. coli) | 4 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 3 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Vis. Sci., 40, 1071-1080 (1999). |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Kubo Y, Arima M, Nakao S, Hisatomi T, Ikeda Y, Matsuda A, Sonoda KH, Ishibashi T. Tenascin-C secreted by transdifferentiated retinal pigment epithelial cells promotes choroidal neovascularization via integrin αV. Lab Invest 96(11) 1178-1188(2016) 27668890 |