Strain Data Sheet

RBRC00066

Strain Information

Image
BRC No.RBRC00066
TypeTargeted Mutation and Spontaneous MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Tnc<tm1Sia> Reln<rl-Orl>/Rbrc
Former Common nameBALB/c-Reln<rl>/+ -TgH(Tnc), TN-reeler, BALB/cA-Reln<rl-Orl>/+ -TgH(Tnc)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cA
Appearance
Strain developmentパスツール研究所より導入したオルレアンリーラーをBALB/c-Tnc<tm1>に導入したダブルミュータント。開発者：日下部守昭先生。reeler ホモ個体は運動障害、歩行異常、成長不良なのでヘテロで維持。繁殖に使えるホモ個体もある。精子、卵子は正常なものが採取可能なので体外受精による個体作出ができる。Tncはホモ欠損で維持する。兄妹交配。繁殖効率はA。
Strain descriptionReelinはEGF様モチーフをもつ巨大な分泌型細胞外蛋白で小脳の顆粒神経細胞、大脳では初期のパイオニア神経細胞に強く発現し、神経組織の層構築に重要な分子である。一方、テネイシンも同じくEGF様モチーフを持つ細胞外マトリックス蛋白質で両者の組織分布は重複する部分が大きい。こうした細胞外蛋白の神経系機能における役割の解析に有用である。
Colony maintenance
References
Murine tenascin: cDNA cloning, structure and temporal expression of isoforms.
Y Saga, T Tsukamoto, N Jing, M Kusakabe, T Sakakura
Gene, 104, 177-185 (1991). 1717349

Mice develop normally without tenascin.
Y Saga, T Yagi, Y Ikawa, T Sakakura, S Aizawa
Genes Dev., 6, 1821-1831 (1992). 1383086

The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.
S Hirotsune, T Takahara, N Sasaki, K Hirose, A Yoshiki, T Ohashi, M Kusakabe, Y Murakami, M Muramatsu, S Watanabe
Nat. Genet., 10, 77-83 (1995). 7647795

Tenascin knockout mice: barrels, boundary molecules, and glial scars.
D A Steindler, D Settles, H P Erickson, E D Laywell, A Yoshiki, A Faissner, M Kusakabe
J. Neurosci., 15, 1971-1983 (1995). 7534342

Abnormal behavior and neurotransmissions of tenascin gene knockout mouse.
F Fukamauchi, N Mataga, Y J Wang, S Sato, A Youshiki, M Kusakabe
Biochem. Biophys. Res. Commun., 221, 151-156 (1996). 8660327

Dysfunction of the Orleans reeler gene arising from exon skipping due to transposition of a full-length copy of an active L1 sequence into the skipped exon.
T Takahara, T Ohsumi, J Kuromitsu, K Shibata, N Sasaki, Y Okazaki, H Shibata, S Sato, A Yoshiki, M Kusakabe, M Muramatsu, M Ueki, K Okuda, Y Hayashizaki
Hum. Mol. Genet., 5, 989-993 (1996). 8817336

Tyrosine hydroxylase activity and its mRNA level in dopaminergic neurons of tenascin gene knockout mouse.
F Fukamauchi, N Mataga, Y J Wang, S Sato, A Yoshiki, M Kusakabe
Biochem. Biophys. Res. Commun., 231, 356-359 (1997). 9070278

Cerebellar histogenesis as seen in identified cells of normal-reeler mouse chimeras.
A Yoshiki, M Kusakabe
Int. J. Dev. Biol., 42, 695-700 (1998). 9712524

Loss of cortical and thalamic neuronal tenascin-C expression in a transgenic mouse expressing exon 1 of the human Huntington disease gene.
M Kusakabe, L Mangiarini, E D Laywell, G P Bates, A Yoshiki, N Hiraiwa, J Inoue, D A Steindler
J. Comp. Neurol., 430, 485-500 (2001). 11169482

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnc
MGI:101922		tenascin C4Tnc<tm1Sia>
MGI:2148540		targeted mutation 1, Shinichi Aizawa
  • autosomal dominant nonsyndromic hearing loss 56(MedGEN)
    • lacZ beta-galactosidase (E. coli)4
    neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)
    Reln
    MGI:103022    		reelin5Reln<rl-Orl>
    MGI:1856416    		reeler Orleans
  • epilepsy, familial temporal lobe, 1(MedGEN)

  • familial temporal lobe epilepsy 7(MedGEN)

  • Norman-Roberts syndrome(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal CNS synaptic transmission (MP:0002206)

  • abnormal circadian behavior (MP:0020467)

  • abnormal olfactory bulb granule cell layer morphology (MP:0009949)

  • abnormal olfactory bulb morphology (MP:0000819)

  • abnormal postnatal subventricular zone morphology (MP:0004275)
    • more 7 phenotypes
  • abnormal rostral migratory stream morphology (MP:0004279)

  • abnormal serotonin level (MP:0005322)

  • decreased dopamine level (MP:0005643)

  • hyperactivity (MP:0001399)

  • impaired swimming (MP:0001522)

  • increased stereotypic behavior (MP:0001409)

  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse tenascin genomic DNA
    Research applicationMouse Models for Human Disease
    Neurobiology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Hum. Mol. Genet., 5, 989-993 (1996). Biochem. Biophys. Res. Commun., 221, 151-156 (1996). J. Neuroscience, 15, 1971-1983 (1995).
    DepositorRIKEN BRC（独立行政法人理化学研究所）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data