Strain Data Sheet

RBRC00045

Strain Information

Image
BRC No.RBRC00045
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameB6C3Fe-a/a-Reln<rl>
Former Common name
H-2 Haplotype
ES Cell line
Background strainmixed background of B6 and C3H.
Appearanceblack, unaffected [a/a +/? or a/a Relnrl/+]
black, ataxic [a/a Relnrl/Relnrl]
Strain developmentThis substrain of reeler mice were developed by Ogawa M (Neuron14, 1995) by extensive selection ofhomozygous breeders from original mutant mice. The strain has been maintained by sister brother mating for more than15 generations in RIKEN Tsukuba Institute.
Strain descriptionThe reeler mutation was first identified by Falconer as neurological mutant mice with abnormal locomotion (J Genet 50, 192-201, 1951) . Laminar formation of cerebral and cerebellar cortex was severely impaired in homozygous mutant mice. This mutant lacks entire Relin gene associated with large deletion of about 150kb.
Colony maintenanceHeterozygous females x homozygous males, or heterozygote x heterozygote. Some homozygous females may also be used as breeders.
References
The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons.
M Ogawa, T Miyata, K Nakajima, K Yagyu, M Seike, K Ikenaka, H Yamamoto, K Mikoshiba
Neuron, 14, 899-912 (1995). 7748558

The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.
S Hirotsune, T Takahara, N Sasaki, K Hirose, A Yoshiki, T Ohashi, M Kusakabe, Y Murakami, M Muramatsu, S Watanabe
Nat. Genet., 10, 77-83 (1995). 7647795

Reelin controls position of autonomic neurons in the spinal cord.
J W Yip, Y P Yip, K Nakajima, C Capriotti
Proc. Natl. Acad. Sci. USA, 97, 8612-8616 (2000). 10880573

Autosomal recessive lissencephaly with cerebellar hypoplasia is associated with human RELN mutations.
S E Hong, Y Y Shugart, D T Huang, S A Shahwan, P E Grant, J O Hourihane, N D Martin, C A Walsh
Nat. Genet., 26, 93-96 (2000). 10973257

Cortical upper layer neurons derive from the subventricular zone as indicated by Svet1 gene expression.
V Tarabykin, A Stoykova, N Usman, P Gruss
Development, 128, 1983-1993 (2001). 11493521

The reelin pathway modulates the structure and function of retinal synaptic circuitry.
D S Rice, S Nusinowitz, A M Azimi, A Martínez, E Soriano, T Curran
Neuron, 31, 929-941 (2001). 11580894

Down-regulation of dendritic spine and glutamic acid decarboxylase 67 expressions in the reelin haploinsufficient heterozygous reeler mouse.
W S Liu, C Pesold, M A Rodriguez, G Carboni, J Auta, P Lacor, J Larson, B G Condie, A Guidotti, E Costa
Proc. Natl. Acad. Sci. USA, 98, 3477-3482 (2001). 11248103

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:G囮検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
a
MGI:87853		nonagouti2a<a>
MGI:1855937		nonagouti
Reln
MGI:103022		reelin5Reln<rl>
MGI:1856398		reeler
  • epilepsy, familial temporal lobe, 1(MedGEN)

  • familial temporal lobe epilepsy 7(MedGEN)

  • Norman-Roberts syndrome(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • Purkinje cell degeneration (MP:0000876)

  • abnormal cerebellum morphology (MP:0000849)

  • abnormal cerebral cortex morphology (MP:0000788)

  • abnormal cortical marginal zone morphology (MP:0000792)

  • abnormal dentate gyrus morphology (MP:0000812)
    • more 35 phenotypes
  • abnormal entorhinal cortex morphology (MP:0006300)

  • abnormal gait (MP:0001406)

  • abnormal hippocampus CA1 region morphology (MP:0008263)

  • abnormal hippocampus CA2 region morphology (MP:0008265)

  • abnormal hippocampus morphology (MP:0000807)

  • abnormal hippocampus pyramidal cell layer (MP:0008284)

  • abnormal neuron differentiation (MP:0009937)

  • abnormal neuronal migration (MP:0006009)

  • abnormal olfactory cortex morphology (MP:0005267)

  • abnormal prepulse inhibition (MP:0003088)

  • abnormal sensory neuron innervation pattern (MP:0000968)

  • abnormal sexual interaction (MP:0002566)

  • abnormal short-term spatial reference memory (MP:0008431)

  • abnormal social investigation (MP:0001360)

  • abnormal stratification in cerebral cortex (MP:0000790)

  • abnormal ventral striatum morphology (MP:0004103)

  • ataxia (MP:0001393)

  • decreased Purkinje cell number (MP:0000880)

  • decreased body size (MP:0001265)

  • decreased body weight (MP:0001262)

  • decreased dopaminergic neuron number (MP:0011448)

  • decreased locomotor activity (MP:0001402)

  • diarrhea (MP:0005036)

  • disheveled coat (MP:0001511)

  • impaired balance (MP:0001525)

  • impaired righting response (MP:0001523)

  • infertility (MP:0001924)

  • lethality at weaning, complete penetrance (MP:0011083)

  • male infertility (MP:0001925)

  • postnatal growth retardation (MP:0001732)

  • premature death (MP:0002083)

  • reduced female fertility (MP:0001923)

  • slow postnatal weight gain (MP:0008489)

  • small cerebellum (MP:0000852)

  • tremors (MP:0000745)
    • Detailed phenotype data

    Ordering Information

    供与核酸
    Research applicationDevelopmental Biology Research
    Mouse Models for Human Disease
    Neurobiology Research
    提供条件条件を付加しない。
    Depositor小川　正晴（理化学研究所脳科学総合研究センター）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Nishibe M, Toyoda H, Hiraga SI, Yamashita T, Katsuyama Y.
    Synaptic and Genetic Bases of Impaired Motor Learning Associated with Modified Experience-Dependent Cortical Plasticity in Heterozygous Reeler Mutants.
    Cereb Cortex 32(3) 504-519(2022) 34339488
    Nishibe M, Katsuyama Y, Yamashita T.
    Developmental abnormality contributes to cortex-dependent motor impairments and higher intracortical current requirement in the reeler homozygous mutants.
    Brain Struct Funct 223(6) 2575-2587(2018) 29536172