Strain Data Sheet
RBRC00045
Strain Information | |
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| Image |  |
| BRC No. | RBRC00045 |
| Type | Spontaneous Mutation |
| Species | Mus musculus |
| Strain name | B6C3Fe-a/a-Reln<rl> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | mixed background of B6 and C3H. |
| Appearance | black, unaffected [a/a +/? or a/a Relnrl/+] black, ataxic [a/a Relnrl/Relnrl] |
| Strain development | This substrain of reeler mice were developed by Ogawa M (Neuron14, 1995) by extensive selection ofhomozygous breeders from original mutant mice. The strain has been maintained by sister brother mating for more than15 generations in RIKEN Tsukuba Institute. |
| Strain description | The reeler mutation was first identified by Falconer as neurological mutant mice with abnormal locomotion (J Genet 50, 192-201, 1951) . Laminar formation of cerebral and cerebellar cortex was severely impaired in homozygous mutant mice. This mutant lacks entire Relin gene associated with large deletion of about 150kb. |
| Colony maintenance | Heterozygous females x homozygous males, or heterozygote x heterozygote. Some homozygous females may also be used as breeders. |
| References | The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons. S Hirotsune, T Takahara, N Sasaki, K Hirose, A Yoshiki, T Ohashi, M Kusakabe, Y Murakami, M Muramatsu, S Watanabe Nat. Genet., 10, 77-83 (1995). 7647795The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons. M Ogawa, T Miyata, K Nakajima, K Yagyu, M Seike, K Ikenaka, H Yamamoto, K Mikoshiba Neuron, 14, 899-912 (1995). 7748558Autosomal recessive lissencephaly with cerebellar hypoplasia is associated with human RELN mutations. S E Hong, Y Y Shugart, D T Huang, S A Shahwan, P E Grant, J O Hourihane, N D Martin, C A Walsh Nat. Genet., 26, 93-96 (2000). 10973257Reelin controls position of autonomic neurons in the spinal cord. J W Yip, Y P Yip, K Nakajima, C Capriotti Proc. Natl. Acad. Sci. USA, 97, 8612-8616 (2000). 10880573The reelin pathway modulates the structure and function of retinal synaptic circuitry. D S Rice, S Nusinowitz, A M Azimi, A Martínez, E Soriano, T Curran Neuron, 31, 929-941 (2001). 11580894Cortical upper layer neurons derive from the subventricular zone as indicated by Svet1 gene expression. V Tarabykin, A Stoykova, N Usman, P Gruss Development, 128, 1983-1993 (2001). 11493521Down-regulation of dendritic spine and glutamic acid decarboxylase 67 expressions in the reelin haploinsufficient heterozygous reeler mouse. W S Liu, C Pesold, M A Rodriguez, G Carboni, J Auta, P Lacor, J Larson, B G Condie, A Guidotti, E Costa Proc. Natl. Acad. Sci. USA, 98, 3477-3482 (2001). 11248103 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| a MGI:87853 | nonagouti | 2 | a<a> MGI:1855937 | nonagouti | |||
| Reln MGI:103022 | reelin | 5 | Reln<rl> MGI:1856398 | reeler | |||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | more 35 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | |
| Research application | Developmental Biology Research Mouse Models for Human Disease Neurobiology Research |
| Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
| Depositor | Masaharu Ogawa (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Nishibe M, Toyoda H, Hiraga SI, Yamashita T, Katsuyama Y. Synaptic and Genetic Bases of Impaired Motor Learning Associated with Modified Experience-Dependent Cortical Plasticity in Heterozygous Reeler Mutants. Cereb Cortex 32(3) 504-519(2022) 34339488 |
Nishibe M, Katsuyama Y, Yamashita T. Developmental abnormality contributes to cortex-dependent motor impairments and higher intracortical current requirement in the reeler homozygous mutants. Brain Struct Funct 223(6) 2575-2587(2018) 29536172 |