Strain Data Sheet

RBRC00041

Strain Information

Image
BRC No.RBRC00041
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC;B6-a/a-Tyrp1<b>-Kit<W-sh>/Rbrc
Former Common nameC;B6-a/a・b/b-WSh/+, Sash brown, B6C3-WSh/+ (brown)
H-2 Haplotype
ES Cell line
Background strain
Appearancebrown
Strain developmentC57BL/6-W<Sh>/+を大阪大学北村幸彦先生より導入。W遺伝子のメラノサイトにおける機能解析のためにBALB/c系統に戻し交配して毛色茶の系統を作出した。開発者:日下部守昭先生兄妹交配による維持。ヘテロ個体と野生型との交配、または、ヘテロ個体同士の交配により維持する。ホモ個体の雄は不妊。繁殖効率：A
Strain descriptionSash semidominant遺伝子を有するミュータント。c-kit遺伝子の機能解析。メラノサイトの発生学的解析。ヘテロ個体は腰部に白班。ホモ個体は黒目の毛白。ホモ個体の雄は妊性有り。
Colony maintenance
References
c-kit Gene was not transcribed in cultured mast cells of mast cell-deficient Wsh/Wsh mice that have a normal number of erythrocytes and a normal c-kit coding region.
T Tono, T Tsujimura, U Koshimizu, T Kasugai, S Adachi, K Isozaki, S Nishikawa, M Morimoto, Y Nishimune, S Nomura
Blood, 15, 1448-1453 (1992). 1381627

W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis.
R Duttlinger, K Manova, T Y Chu, C Gyssler, A D Zelenetz, R F Bachvarova, P Besmer
Development, 118, 705-717 (1993) 7521281

C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression.
M Yamazaki, T Tsujimura, E Morii, K Isozaki, H Onoue, S Nomura, Y Kitamura
Blood, 83, 3509-3516 (1994). 7515715

The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.
G Berrozpe, I Timokhina, S Yukl, Y Tajima, M Ono, A D Zelenetz, P Besmer
Blood, 94, 2658-2666 (1999). 10515869

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Michele A Grimbaldeston, Ching-Cheng Chen, Adrian M Piliponsky, Mindy Tsai, See-Ying Tam, Stephen J Galli
Am. J. Pathol., 167, 835-848 (2005) 16127161

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
a
MGI:87853		nonagouti2a<a>
MGI:1855937		nonagouti
Tyrp1
MGI:98881		tyrosinase-related protein 14Tyrp1<b>
MGI:1855960		brown
  • oculocutaneous albinism type 3(MedGEN)
    • Kit
    MGI:96677    		KIT proto-oncogene receptor tyrosine kinase5Kit<W-Sh>
    MGI:1856265    		sash
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal coat/hair pigmentation (MP:0002075)

  • abnormal definitive hematopoiesis (MP:0002123)

  • abnormal erythrocyte cell number (MP:0001586)

  • abnormal vascular permeability (MP:0001619)

  • absent coat pigmentation (MP:0005171)
    • more 7 phenotypes
  • decreased erythrocyte cell number (MP:0002875)

  • decreased mast cell degranulation (MP:0008765)

  • decreased mast cell number (MP:0000336)

  • decreased susceptibility to type I hypersensitivity reaction (MP:0005597)

  • impaired behavioral response to xenobiotic (MP:0009747)

  • increased susceptibility to parasitic infection (MP:0005027)

  • variable body spotting (MP:0002940)
    • Detailed phenotype data

    Ordering Information

    供与核酸
    Research application
    提供条件条件を付加しない。
    DepositorRIKEN BRC（独立行政法人理化学研究所）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data