Strain Data Sheet

RBRC00040

Strain Information

Image
BRC No.RBRC00040
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC;B6 a-Kit<W-sh>/Rbrc
Former Common nameC;B6-a/a・B/B-W Sh/+, Sash black, B6C3-W Sh/+ (black)
H-2 Haplotype
ES Cell line
Background strain
Appearancewhite spot [Sh hetero]
black eyed, white coat, affected [Sh homo]
Strain developmentC57BL/6-WSh/+ was gifted from Dr. Yukihiko Kitamura, Osaka University to Dr. Kusakabe M, RIKEN Tsukuba Institute.
Strain descriptionHomozygotes are viable, males are sterile. Sash semidominannt
Colony maintenance
References
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Grimbaldeston M A, Chen C C, Piliponsky A M, Tsai M, Tam S Y, Galli S J
Am. J. Pathol., 167, 835-848 (2005) 16127161

C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression.
Yamazaki M, Tsujimura T, Morii E, Isozaki K, Onoue H, Nomura S, Kitamura Y
Blood, 83, 3509-3516 (1994). 7515715

W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis.
Duttlinger R, Manova K, Chu T Y, Gyssler C, Zelenetz A D, Bachvarova R F, Besmer P
Development, 118, 705-717 (1993). 7521281

The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.
Berrozpe G, Timokhina I, Yukl S, Tajima Y, Ono M, Zelenetz A D, Besmer P
Blood, 94, 2658-2666 (1999). 10515869

c-kit Gene was not transcribed in cultured mast cells of mast cell-deficient Wsh/Wsh mice that have a normal number of erythrocytes and a normal c-kit coding region.
Tono T, Tsujimura T, Koshimizu U, Kasugai T, Adachi S, Isozaki K, Nishikawa S, Morimoto M, Nishimune Y, Nomura S, et a l
Blood, 15, 1448-1453 (1992). 1381627

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
anonagouti2anonagouti
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
  • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)
  • KitKIT proto-oncogene receptor tyrosine kinase5Kitsash
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
  • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal definitive hematopoiesis(MP:0002123)

  • abnormal erythrocyte cell number(MP:0001586)

  • abnormal vascular permeability(MP:0001619)

  • absent coat pigmentation(MP:0005171)
  • more 8 phenotypes
  • belly spot(MP:0000373)

  • decreased erythrocyte cell number(MP:0002875)

  • decreased mast cell degranulation(MP:0008765)

  • decreased mast cell number(MP:0000336)

  • decreased susceptibility to type I hypersensitivity reaction(MP:0005597)

  • impaired behavioral response to xenobiotic(MP:0009747)

  • increased susceptibility to parasitic infection(MP:0005027)

  • variable body spotting(MP:0002940)
  • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data