Strain Data Sheet

RBRC-AES1237

Information1

Image
BRC No.RBRC-AES1237
TypeGene TrapCartagena
SpeciesMus musculus
Strain name26v-018
Former Common nameUbxd4 Gt/#26v-018
H-2 Haplotype
ES Cell line(C57BL/6J x 129/SvJae)F1 embryonic stem cell
Background strain
Appearance
Strain developmentDeveloped by Dr. Yasumasa Ishida, Nara Institute of Science and Technology. v6.4 ES cells derived from (129S4/SvJae x C57BL/6J)F1 were used.
Strain descriptionGene trap ES cell lines using UPATrap method developed by Dr. Yasumasa Ishida, Nara Institute of Science and Technology. UPATrap suppresses a nonsense-mediated mRNA decay of the selectable-marker mRNA and permits the trapping of transcriptionally silent genes without a bias in the vector-integration site. These cells can be used in the in vitro differentiation study. In addition, gene inactivated mouse line can be generated by using these cell clones. Insertion of gene trap vector: pUPAT-egfp3ic
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ubxd4UBX domain protein 41Ubxd4<Gt(NAISTrap_26v1018)Yais>

Information2

Donor DNAP1 Phage loxP, yeast FRT (flipase recombination target) sites, Jellyfish EGFP (green fluorescence protein) cDNA, mouse hypoxanthine-guanine phosphoribosyl transferase gene (hprt) genomic DNA, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), LTR (long terminal repeat), SA (splice acceptor), CP (constitutive promoter)
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions(1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. When the RECIPIENT use the BIOLOGICAL RESOURCE in collaboration with a for-profit organization, it is recognized as a for-profit utilization and the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
(2) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
(3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Yasumasa Ishida ) is requested.
(4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of a paper (Nucleic Acids Res. 2005;33:e20) is requested.
(5) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(6) When the RECIPIENT established a mouse line by using of the BIOLOGICAL RESOURCE, the RECIPIENT must deposit the mouse line to the Experimental Animal Division of RIKEN BioResource Center.
DepositorYasumasa Ishida (Nara Institute of Science and Technology)
Strain StatusES cell lines
Strain AvailabilityChimeric mice from blastocyst injection of ES cell clone (more than 3 months)
Additional Info.Please contact "cellqa@brc.riken.jp" for distribution.
DDBJ: CZ905187
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data