RBRC10171

Information1

Image
BRC No.RBRC10171
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common nameRosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentインジェクションの受精卵系統:C57BL/6ジーンターゲティングに用いたES細胞名:V6.5開発者:柴田博史開発年:2015ヘテロとWTの交配により系統を維持
Strain description組み換え酵素Creが発現した細胞でrtTA3の発現誘導が可能さらにDoxycycline 投与により、rtTA3が発現した細胞でOSKM, mCherry の発現誘導が可能。
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack2020/07/06Room:3-3Rack:D
2020/04/06Room:3-3Rack:D
2020/02/03Room:3-3Rack:D
2019/12/02Room:3-3Rack:D
2019/10/16Room:3-3Rack:D
2019/08/05Room:3-3Rack:D
2019/06/03Room:3-3Rack:D
2019/04/01Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Col1a1collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>targeted mutation 4, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu>targeted mutation 3, Yasuhiro Yamada

Information2

供与核酸adenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Commun., 9, 2081 (2018).
学術機関の学術研究に限る。
Depositor山田 泰広(国立大学法人京都大学)
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-
Category I MTA: FP license of TBUSA

BRC mice in Publications

No Data