RBRC10145

Information1

Image
BRC No.RBRC10145
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tppp3<tm1(EGFP)Yasu>
Former Common nameTppp3-EGFP
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentインジェクションの受精卵系統:B6D2F1ジーンターゲティングに用いたES細胞名:V6.5開発者:河村真吾開発年:2013ホモ同士の交配により系統を維持。
Strain descriptionTppp3が発現する細胞でEGFPが発現する。
Colony maintenance
ReferencesNat. Commun., 10(1):3999 (2019). 31488818
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. (in revision)

Health Report

Examination Date / Room / Rack2020/02/17Room:3-DRack:C
2019/12/16Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tppp3tubulin polymerization-promoting protein family member 38Tppp3<tm1(EGFP)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)8Bsdmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA8HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site8rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
roxD6 Bacteriophage rox site8rox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal8

Information2

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Aequorea victoria EGFP cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat Commun. 2019 Sep 5;10(1):3999.
学術機関の学術研究に限る。
Depositor山田 泰広(国立大学法人京都大学)
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data