RBRC02463

Information1

Image
BRC No.RBRC02463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nefh<tm1(Venus)Ito>
Former Common nameNF-H KO mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development開発者/機関:神吉浩明先生、糸原重美先生/理研脳センター開発年:2003年
Strain descriptionNF-H遺伝子座Exon1のNotI siteにVenus遺伝子を挿入してある。蛍光蛋白質Venusの発現は検出されない。選択マーカー (pgk-neo) は除去済みである。C57BL/6 isogene.
Colony maintenance繁殖効率良好
References

Health Report

Examination Date / Room / Rack2008/09/08Room:4-5Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nefhneurofilament, heavy polypeptide11Nefh<tm1(Venus)Ito>targeted mutation 1 (venus), Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)11Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
myc-tagmyc-epitope tag (rat)11myc-tag

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Information2

供与核酸Phage P1 LoxP site, Jellyfish Venus cDNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), yeast FRT site, rat Myc-epitope, bovine BGH poly A, mouse Nefh genomic DNA
Research applicationNeurobiology Research
提供条件条件を付加しない。
Depositor糸原 重美(独立行政法人理化学研究所)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data