RBRC02350

Information1

Image
BRC No.RBRC02350
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Slc1a3<tm1(crePR)Ksak>
Former Common nameGLAST-CrePR
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development新潟大学脳研究所・辻田実加、夏目里恵、崎村建司(2004)。C57BL/6NCrj由来のRENKA ES細胞株を用いて作製。C57BL/6N背景。
Strain descriptionグルタミン酸トランスポーター遺伝子Slc1a3のCrePRノックインマウス。Slc1a3 (GLAST)遺伝子の開始メチオニンからインフレームでCrePR配列を挿入。CrePRの下流にG-CSF3’ non-cordingとpgk-neoカセットをもつ。pgk-neoカセットは両側にfrt配列をもち、FLPリコンビネースにより除去が可能。
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Slc1a3Progesterone receptor15Slc1a3<tm1(crePR)Ksak>targeted mutation 1 (CrePR), Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
PGRProgesterone receptor (human)15PGR

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase15cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Amouse pgk polyA15poly A

Information2

供与核酸mouse phosphoglycerokinase(pgk) promoter, mouse pgk poly A, E. coli neo, Bacteriophage P1 Cre, Human progesterone receptor B, S. cerevisiae 2m plasmid FRT
Research applicationCre/loxP system
Neurobiology Research
General Purpose
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Depositor崎村 建司(国立大学法人新潟大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data