RBRC02340

Information1

Image
BRC No.RBRC02340
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(bitetO-GFP)BYoko
Former Common namebitetO-GFP
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development開発者/機関名:横井峰人先生/コロンビア大学開発年:2002年
Strain descriptionテトラサイクリン依存性プロモーターにより発現調節を受け、細胞全体が緑色蛍光にて可視化できるトランスジェニックマウス。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg/+ hemizygous mice are viable and fertile.
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2010/11/16Room:4-ARack:E
2010/09/13Room:4-ARack:E
2010/07/12Room:4-ARack:E
2010/05/17Room:4-ARack:E
2010/03/15Room:4-ARack:E
2010/01/18Room:4-ARack:E
2009/11/16Room:4-ARack:E
2009/09/14Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gria2glutamate receptor, ionotropic, AMPA2 (alpha 2)UNGria2Glur-2, GluR-B, GluR2, Glur2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptprdprotein tyrosine phosphatase, receptor type, DUNPtprd1110002J03Rik, 3000002J10Rik, B230219D21Rik, MGC:36851

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Jellyfish)UNE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PLAPhuman placental alkaline phosphataseUNPLAP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA signalUNpoly A

Information2

供与核酸E. coli tetO, CMV minimal promoter, Aequoria Victoria GFP cDNA, Rabbit beta-Globin intron/polyA signal, Human placental alkaline phosphatase, Clostridium tetani Non-toxic c fragment, Mouse tyrosine phosphatase δ(fragment), Mouse glutamate receptor 2 (fragment), SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 27, 6948-6955 (2007).
公表を前提とした非営利目的の学術研究での使用に限る。
Depositor横井 峰人(国立大学法人京都大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Mayumi Yamada, Yusuke Suzuki, Shinji C.Nagasaki, Hiroyuki Okuno, Itaru Imayoshi.
Light Control of the Tet Gene Expression System in Mammalian Cells.
Cell Rep 25(2) 487-500.(2018) 30304687