RBRC02291

Information1

Image
BRC No.RBRC02291
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-spH)CYoko
Former Common nameTetO-synaptopHluorin
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain development開発者/機関:横井峰人先生/京都大学開発年:2005年
Strain descriptionテトラサイクリン依存性プロモーターにより発現調節を受け、神経終末が緑色蛍光にて可視化できるトランスジェニックマウス。
Colony maintenanceHemizygote x Wild-type (C57BL/6CrSlc)
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2010/12/13Room:3-CRack:I
2010/10/18Room:3-CRack:I
2010/08/23Room:3-CRack:I
2010/06/21Room:3-CRack:I
2010/04/19Room:3-CRack:I
2010/02/22Room:3-CRack:I
2009/12/14Room:3-CRack:I
2009/10/19Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vamp2vesicle-associated membrane protein 2UNVamp2E. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
pHluorinpH-sensitive green fluorescent proteins, S147E, N149L, I161T, V163A, N164I, K166Q, I167V, R168H, S202H and L231H. (Jellyfish)UNpHluorin

Information2

供与核酸E. coli tetO, CMV minimal promoter, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).), rabbit beta-globin intron/polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
General Purpose
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J.Neurosci., 27, 6948-6955 (2007).
公表を前提とした非営利目的の学術研究での使用に限る。
Depositor横井 峰人(国立大学法人京都大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data