RBRC02290

Information1

Image
BRC No.RBRC02290
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Nanog-GFP,Puro)1Yam
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentRF8(129S4/SvJae由来)ES細胞をC57BL/6JJclのblastocystにインジェクションして作製。C57BL/6Jに数回戻し交配をした後、DBA/2JJclと1回交配を行い、その後、兄妹交配にて維持。
Strain descriptioniPS細胞 (誘導性多能性幹細胞 induced pluripotent stem cell) 作製系統 (Nanog-GFPレポーター系統) 。Tgホモ/ヘミマウスともに生存性、繁殖性あり。性差の偏り (雄の偏り) あり (詳細不明) 。
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote)
ReferencesNature, 448, 313-317 (2007) 17554338

Health Report

Examination Date / Room / Rack2020/11/16Room:3-CRack:K
2020/08/24Room:3-CRack:K
2020/08/17Room:3-CRack:K
2020/06/01Room:3-CRack:K
2020/02/17Room:3-CRack:K
2019/12/16Room:3-CRack:K
2019/12/03Room:3-CRack:K
2019/10/21Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Jellyfish)UNNanog promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin resistance gene (Streptomyces alboniger)UNpuro

Information2

供与核酸Jellyfish EGFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), S. alboniger puromycin resistance gene, bovine growth hormone polyadenylation signal, yeast FRT (flipase recombination target) site, mouse genomic DNA from RP23-117GFPNF
Research applicationCell Biology Research
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件
Depositor山中 伸弥(国立大学法人京都大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Mouse of the Month Dec 2007
Mouse of the Month Oct 2012
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: (Japanese/ English)
  3. Distribution of the iPS cells (Japanese/ English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Nagamatsu G, Kosaka T, Saito S, Takubo K, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T.
Tracing the Conversion Process for Primordial Germ Cells to Pluripotent Stem Cells in Mice.
Biol. Reprod. (2012) 22423052

Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.
Cell Stem Cell 14(2) 217-27(2014) 24506885

Nukaya D, Minami K, Hoshikawa R, Yokoi N, Seino S.
Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas.
Genes Cells 20(5) 367-81(2015) 25727848

Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
A germ cell-specific gene, Prmt5, works in somatic cell reprogramming.
J. Biol. Chem. 286(12) 10641-8(2011) 21270127

Jincho Y, Araki R, Hoki Y, Tamura C, Nakamura M, Ando S, Kasama Y, Abe M.
Generation of Genome Integration-free Induced Pluripotent Stem Cells from Fibroblasts of C57BL/6 Mice without c-Myc Transduction.
J. Biol. Chem. 285(34) 26384-9(2010) 20554535

Koga M, Matsuda M, Kawamura T, Sogo T, Shigeno A, Nishida E, Ebisuya M.
Foxd1 is a mediator and indicator of the cell reprogramming process.
Nat Commun 5 3197(2014) 24496101

Okita K, Hong H, Takahashi K, Yamanaka S.
Generation of mouse-induced pluripotent stem cells with plasmid vectors.
Nat Protoc 5(3) 418-28(2010) 20203661

Cavallari C, Fonsato V, Herrera MB, Bruno S, Tetta C, Camussi G.
Role of Lefty in the anti tumor activity of human adult liver stem cells.
Oncogene 32(7) 819-26(2013) 22469982

Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami KI, Takahashi S, Okuda A, Okazaki Y.
Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells.
Stem Cells (2015) 26523946

Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M.
Crucial Role of c-Myc in the Generation of Induced Pluripotent Stem Cells.
Stem Cells (2011) 21732496

Araki R, Jincho Y, Hoki Y, Nakamura M, Tamura C, Ando S, Kasama Y, Abe M.
Conversion of ancestral fibroblasts to induced pluripotent stem cells.
Stem Cells 28(2) 213-20(2010) 20020427

Swistowski A, Peng J, Liu Q, Mali P, Rao MS, Cheng L, Zeng X.
Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions.
Stem Cells 28(10) 1893-904(2010) 20715183