RBRC02228

Information1

Image
BRC No.RBRC02228
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameTOP-GAL
Former Common name
H-2 Haplotype
ES Cell line
Background strainICR
Appearancealbino [c/c]
Strain development国立遺伝学研究所・相賀裕美子先生(2005)。B6C3F2受精卵へインジェクションにより作出。ICR背景。
Strain descriptionWntシグナルのレポーターであるTOPGalのトランスジェニックマウス。
Colony maintenanceヘテロで維持。
ReferencesGenesis, 45, 90-100 (2007) 17245775

Health Report

Examination Date / Room / Rack2014/08/04Room:3-1Rack:B
2014/06/02Room:3-1Rack:B
2014/05/12Room:3-1Rack:B
2014/03/10Room:2-検Rack:C
2014/01/14Room:2-検Rack:C
2013/11/11Room:2-検Rack:C
2013/09/09Room:2-検Rack:C
2013/07/24Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

供与核酸TCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls)
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
General Purpose
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 45, 90-100 (2007).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor相賀 裕美子(大学共同利用機関法人情報・システム研究機構国立遺伝学研究所)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Saga Lab HP
Genotyping protocol -PCR-
Mouse of the Month Aug 2009
Genetic Background

BRC mice in Publications

Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R.
Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after -irradiation through Wnt/ -catenin pathway.
Carcinogenesis (2015) 25827435

Garbe DS, Ring RH.
Investigating Tonic Wnt Signaling Throughout the Adult CNS and in the Hippocampal Neurogenic Niche of BatGal and Ins-TopGal Mice.
Cell. Mol. Neurobiol. (2012) 22491991

Lu TL, Chen CM.
Differential requirements for beta-catenin in murine prostate cancer originating from basal versus luminal cells.
J. Pathol. 236(3) 290-301(2015) 25712462

Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
Sci Rep 4 6959(2014) 25376241