RBRC01834

Information1

Image
BRC No.RBRC01834
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc
Former Common nameB6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development理化学研究所脳科学総合研究センター・神吉浩明先生、糸原重美先生(2006)。C57BL/6受精卵へTgのインジェクションにより作出。C57BL/6J背景。
Strain descriptionCAGプロモーター制御下でFLPeを発現するトランスジェニックマウス。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesExp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack2020/07/06Room:4-ARack:F
2020/03/30Room:4-ARack:F
2020/02/07Room:4-ARack:F
2020/01/27Room:4-ARack:F
2020/01/20Room:4-ARack:F
2019/11/25Room:4-ARack:F
2019/10/21Room:4-ARack:F
2019/10/21Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLPeyeast flippase recombinaseUNFLPeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)UNpuro

Information2

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), yeast FLPe cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene
Research applicationFLP/frt system
Genetics Research
General Purpose
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Experimental Animals, 55, 137-141 (2006).
Depositor糸原 重美(独立行政法人理化学研究所)
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Feb 2007
Genetic Background

BRC mice in Publications

Yanagihara T, Tomino T, Uruno T, Fukui Y.
Thymic epithelial cell-specific deletion of Jmjd6 reduces Aire protein expression and exacerbates disease development in a mouse model of autoimmune diabetes.
Biochem. Biophys. Res. Commun. 489(1) 8-13(2017) 28546003

Sugimoto M, Shimizu Y, Zhao S, Ukon N, Nishijima K, Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Okuda T, Tamaki N, Hanamatsu H, Igarashi Y, Kuge Y.
Characterization of the role of sphingomyelin synthase 2 in glucose metabolism in whole-body and peripheral tissues in mice.
Biochim. Biophys. Acta (2016) 27151272

Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H.
Acquired expression of Cbl(Q367P) in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia.
Blood 129(15) 2148-2160(2017) 28209720

Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
Cancer Res. 70(18) 7073-83(2010) 20823158

Nakahara R, Kawai Y, Oda A, Nishimura M, Murakami A, Azuma T, Kaifu T, Goitsuka R.
Generation of a Tlx1CreER-Venus knock-in mouse strain for the study of spleen development.
Genesis (2014) 25283275

Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA.
KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity.
Immunity (2018) 29625895

Okuma A, Hoshino K, Ohba T, Fukushi S, Aiba S, Akira S, Ono M, Kaisho T, Muta T.
Enhanced apoptosis by disruption of the STAT3-IkappaB- signaling pathway in epithelial cells induces Sj gren's syndrome-like autoimmune disease.
Immunity 38(3) 450-60(2013) 23453632

Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
Zfat expression in ZsGreen reporter gene knock?in mice: Implications for a novel function of Zfat in definitive erythropoiesis.
Int. J. Mol. Med. (2018) 30106088

Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T.
Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock.
J. Biol. Chem. 286(37) 32542-51(2011) 21778228

Fumoto T, Takeshita S, Ito M, Ikeda K.
Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis.
J. Bone Miner. Res. (2013) 24014480

Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, Nagashima T, Takahashi Y, Kurosaki T, Okada M, Ohara O, Rajewsky K, Takemori T.
Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.
J. Exp. Med. (2012) 23027924

Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T.
Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling.
J. Invest. Dermatol. 132(5) 1338-45(2012) 22297635

Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M.
Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin.
J. Neurosci. 37(12) 3181-3191(2017) 28213441

Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway.
J. Pathol. (2016) 26913567

Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
Life Sci Alliance (2018) 30456378

Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N.
The histone demethylase fbxl11/kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.
Mech. Dev. (2014) 25463925

Kimura-Yoshida C, Mochida K, Nakaya MA, Mizutani T, Matsuo I.
Cytoplasmic localization of GRHL3 upon epidermal differentiation triggers cell shape change for epithelial morphogenesis.
Nat Commun 9(1) 4059(2018) 30283008

Raveney BJ, Oki S, Hohjoh H, Nakamura M, Sato W, Murata M, Yamamura T.
Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation.
Nat Commun (2015) 26436530

Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.
Nat. Med. 18(3) 405-12(2012) 22344299

Kawai Y, Oda A, Kanai Y, Goitsuka R.
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
PLoS ONE (2018) 29293683

Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H.
Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.
PLoS ONE (2014) 24498102

Ikeda K, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, Takubo K, Kanai A, Oda H, Wolff L, Honda Z, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H.
Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency.
Sci Rep Jul 19;6:29454. doi: 10.1038/srep29454. (2016) 27432459