RBRC01351

Information1

Image
BRC No.RBRC01351
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S-Grm2<tm1Nak>/NakRbrc
Former Common nameC57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentC57BL/6系統(substrain不明)で8世代、その後C57BL/6N(チャールズリバー)で12世代戻し交配を行った。
Strain descriptionmGluR2ノックアウトマウス。コカインへの依存行動が亢進している。行動の異常がある
Colony maintenance繁殖効率A特になし
ReferencesProc. Natl. Acad. Sci. USA, 102, 4170-4175 (1996). 15753323
Science, 273, 645-647 (1996). 8662555

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Grm2G protein-coupled receptor, family C, group 1, member B9Grm2<tm1Nak>targeted mutation 1, Shigetada Nakanishi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

供与核酸mouse glutamate receptor metabotropic 2 genomic DNA, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research applicationNeurobiology Research
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).
研究成果の公表にあたって謝辞の表明を必要とする。
必ず提供承諾書を必要とする。
Depositor中西 重忠(国立大学法人京都大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, Mackerell AD Jr, Brezina V, Sealfon SC, Filizola M, Gonz lez-Maeso J, Logothetis DE.
Decoding the Signaling of a GPCR Heteromeric Complex Reveals a Unifying Mechanism of Action of Antipsychotic Drugs.
Cell 147(5) 1011-23(2011) 22118459

Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cu llar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, L pez-Gim nez JF, Meana JJ, Benson DL, Gonz lez-Maeso J.
Identification of Three Residues Essential for 5-Hydroxytryptamine 2A-Metabotropic Glutamate 2 (5-HT2A{middle dot}mGlu2) Receptor Heteromerization and Its Psychoactive Behavioral Function.
J. Biol. Chem. 287(53) 44301-19(2012) 23129762

Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL.
Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors.
Mol. Neurobiol. (2016) 27878759

Moreno JL, Holloway T, Rayannavar V, Sealfon SC, Gonz lez-Maeso J.
Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice.
Neurosci. Lett. (2013) 23333599

Moreno JL, Holloway T, Albizu L, Sealfon SC, Gonz lez-Maeso J.
Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists.
Neurosci. Lett. 493(3) 26542(2011) 21276828

Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J.
Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.
Sci Signal 9(410) ra5. (2016) 26758213