RBRC00522

Information1

Image
BRC No.RBRC00522
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rag2tm1Fwa-IL2rbtm1Mak
Former Common nameC57BL/6-Rag2tm1Fwa-IL2rbtm1Och
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain development1998 by Koyasu at Keio Univ. School of Med.Original :Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USAIL2rbtm1Mak:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, CanadaB6.129-Rag2tm1Fwa  (N10)×B6.129-IL2rbtm1Mak (N8)→F6-7
Strain descriptionThey lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects.
Colony maintenanceNothing special. Breed well.
ReferencesEur. J. Immunol., 31, 3147-3152 (2001). 11745331
Nat. Immunol., 2, 1138-1143 (2001). 11702064
J. Immunol., 157, 4379-4389 (1996). 8906813
Science, 268, 1472-1476 (1995). 7770771
Cell, 68, 855-867 (1992). 1547487

Health Report

Examination Date / Room / Rack2006/04/25Room:2-検Rack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Il2rbinterleukin 2 receptor, beta chain15Il2rb<tm1Mak>targeted mutation 1, Tak W Mak

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rag2recombination activating gene 22Rag2<tm1Fwa>targeted mutation 1, Frederick W Alt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, herpes simplex virus thymidine kinase polyA additional signal, mouse Rag2 genomic DNA, mouse IL2rb genomic DNA
Research applicationCell Biology Research
Immunology and Inflammation Research
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
利用者はnon-profit organizationを対象とし、Dr. Tak W. Makの許可を得ること。
Depositor小安 重夫(慶應義塾大学)
Strain Status凍結胚のアイコン凍結胚
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data