Strain Data Sheet

RBRC00361

Strain Information

Image
BRC No.RBRC00361
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129-Mapk14<tm1Mka>(118)/Rbrc
Former Common nameC57BL/6-TgH(p38)/118
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6J
Appearanceblack [a/a]
Strain developmentThis B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.
Strain descriptionActivity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression.
Colony maintenanceThe mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14).
References
Requirement for p38alpha in erythropoietin expression: a role for stress kinases in erythropoiesis.
Tamura K, Sudo T, Senftleben U, Dadak A M, Johnson R, Karin M
Cell, 102, 221-231(2000). 10943842

Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity.
Takanami-Ohnishi Y, Amano S, Kimura S, Asada S, Utani A, Maruyama M, Osada H, Tsunoda H, Irukayama-Tomobe Y, Goto K, Karin M, Sudo T, Kasuya Y
J. Biol. Chem., 277, 37896-37903 (2002). 12138127

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mapk14mitogen-activated protein kinase 1417Mapk14targeted mutation 1, Michael Karin
neoneomycin resistance gene (E. coli)17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal erythropoiesis(MP:0000245)

  • abnormal placental labyrinth vasculature morphology(MP:0008803)

  • abnormal response to cardiac infarction(MP:0000343)

  • anemia(MP:0001577)

  • decreased myocardial infarct size(MP:0003038)
    • more 7 phenotypes
  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, incomplete penetrance(MP:0011108)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • pale liver(MP:0000603)

  • pale yolk sac(MP:0001722)

  • pallor(MP:0003717)

  • small liver(MP:0000601)
    • Detailed phenotype data

    Ordering Information

    供与核酸E. coli neo, mouse p38 genomic DNA
    Research application
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。研究成果の公表にあたって謝辞の表明を必要とする。公表を前提とした学術研究に限る。共著を必要とする共同研究は寄託後2年間に限定する。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
    Depositor須藤　龍彦（独立行政法人理化学研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ohta M, Tateishi K, Kanai F, Ueha S, Guleng B, Washida M, Tanaka Y, Ijichi H, Ikenoue T, Sata M, Sudo T, Shiina S, Kawabe T, Matsushima K, Omata M.
    Reduced p38 mitogen-activated protein kinase in donor grafts accelerates acute intestinal graft-versus-host disease in mice.
    Eur J Immunol 35(7) 2210-21(2005) 15971269