RBRC00361

Information1

Image
BRC No.RBRC00361
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC57BL/6-TgH(p38)/118
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6J
Appearanceblack [a/a]
Strain developmentThis B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.
Strain descriptionActivity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression.
Colony maintenanceThe mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14).
ReferencesCell, 102, 221-231(2000). 10943842
J. Biol. Chem., 277, 37896-37903 (2002). 12138127

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mapk14mitogen activated protein kinase 1417Mapk14<tm1Mka>targeted mutation 1, Michael Karin

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17

Information2

供与核酸E. coli neo, mouse p38 genomic DNA
Research application
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。
研究成果の公表にあたって謝辞の表明を必要とする。
公表を前提とした学術研究に限る。共著を必要とする共同研究は寄託後2年間に限定する。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
Depositor須藤 龍彦(独立行政法人理化学研究所)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Miki OHTA, Keisuke TATEISHI, Fumihiko KANAI, Satoshi UEHA, Bayasi GULENG, Miwa WASHIDA, Yasuo TANAKA, Hideaki IJICHI, Tsuneo IKENOUE, Masataka SATA, Tatsuhiko SUDO, Shuichiro SHIINA, Takao KAWABE, Kouji MATSUSHIMA, Masao OMATA
Reduced p38 mitogen-activated protein kinase in donor grafts accelerates acute intestinal graft-versus-host disease in mice.
Eur. J. Immunol. 35 2210-2221(2005) 15971269