RBRC00109

Information1

Image
BRC No.RBRC00109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc
Former Common nameC3H/HeN-TgH(Zic1), Zic1 KO (C3H background)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae](D3 subline)
Background strainC3H/HeNJcl
Appearanceagouti
Strain developmentDeveloped by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations.
Strain descriptionThe Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.Genotype of Affected Mutation:Zic1 null heterozygous.
Colony maintenanceBackcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs.
ReferencesMol. Brain Res., 78, 15-25 (2000). 10891581
J. Neurosci., 18, 284-293 (1998). 9412507
Dev. Biol., 244, 329-341 (2002). 11944941
Mech. Dev., 89, 141-150 (1999). 10559489

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zic1zinc finger protein of the cerebellum 19Zic1<tm1Jaru>targeted mutation 1, Jun Aruga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

供与核酸mouse Zic1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationDevelopmental Biology Research
Mouse Models for Human Disease
Neurobiology Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 18, 284-293 (1998).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor有賀 純(理化学研究所脳科学総合研究センター)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Lab HP

BRC mice in Publications

No Data